Effect of cinnamaldehyde treatment on browning and reactive oxygen species metabolism in fresh-cut lettuce

  • XIONG Min ,
  • LIN Desheng ,
  • LIAO Lingyan ,
  • HE Meiying ,
  • LIU Yunfen ,
  • YIN Feilong ,
  • SHUAI Liang
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  • 1(School of Life Science, Nanchang Normal University, Nanchang 330032, China)
    2(School of Food and Bioengineering, Guangxi Key Laboratory of Health Care Food Science and Technology, Hezhou University, Hezhou 542899, China)
    3(School of Food and Pharmaceutical Engineering, Wuzhou University, Wuzhou University, Wuzhou 543002, China)

Received date: 2025-05-21

  Revised date: 2025-07-30

  Online published: 2026-04-10

Abstract

To explore the effect of cinnamaldehyde treatment on browning and active oxygen metabolism of fresh-cut lettuce, this study used fresh-cut lettuce as the raw material and treated it with 0.1, 0.5, and 1.0 g/L cinnamaldehyde under storage conditions of (5±1) ℃ and 85%-90% relative humidity to investigate its effect on browning of fresh-cut lettuce.Results showed that treatment with 1.0 g/L cinnamaldehyde significantly inhibited the increase in browning degree, delayed the accumulation of total phenols, flavonoids, and quinones, and increased the content of ascorbic acid and glutathione.In addition, cinnamaldehyde treatment could inhibit the activity of key enzymes involved in browning, such as polyphenol oxidase and peroxidase, while simultaneously increasing the activity of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase.This enhanced the ability to scavenge reactive oxygen species, thereby reducing oxidative damage and delaying the browning process.In summary, treatment with 1.0 g/L cinnamaldehyde could effectively maintain the storage quality of fresh-cut lettuce, and its mechanism of inhibiting browning by regulating reactive oxygen metabolism provides a new technical approach for the preservation of fresh-cut fruits and vegetables.

Cite this article

XIONG Min , LIN Desheng , LIAO Lingyan , HE Meiying , LIU Yunfen , YIN Feilong , SHUAI Liang . Effect of cinnamaldehyde treatment on browning and reactive oxygen species metabolism in fresh-cut lettuce[J]. Food and Fermentation Industries, 2026 , 52(6) : 164 -172 . DOI: 10.13995/j.cnki.11-1802/ts.043320

References

[1] 杨晓哲, 胡文忠, 姜爱丽, 等.鲜切莴苣生理生化变化及其保鲜技术的研究进展[J].食品与发酵工业, 2018,44(2):278-283.
YANG X Z, HU W Z, JIANG A L, et al. Research progress on physiological and biochemical changes of fresh-cut lettuce and its preservation technology. Food and Fermentation Industries, 2018, 44(2):278-283.
[2] 李静, 季悦, 李美琳, 等.切割方式对鲜切莴苣品质及抗氧化活性的影响[J].食品科学, 2018,39(3):268-273.
LI J, JI Y, LI M L, et al.Effects of cutting methods on quality and antioxidant activity of fresh-cut lettuce[J].Food Science, 2018,39(3):268-273.
[3] 祝竞芳, 汤静, 游万里, 等.氯化钙处理对鲜切莴苣品质及γ-氨基丁酸代谢的影响[J].食品科学, 2022,43(7):174-181.
ZHU J F, TANG J, YOU W L, et al.Effects of calcium chloride treatment on quality and γ-aminobutyric acid metabolism of fresh-cut lettuce[J].Food Science, 2022,43(7):174-181.
[4] NADARAJAH K K.ROS homeostasis in abiotic stress tolerance in plants[J].International Journal of Molecular Sciences, 2020, 21(15):5208.
[5] BLAKEMORE W M, THOMPSON H C Jr.Trace analysis of cinnamaldehyde in animal feed, human urine, and wastewater by electron capture gas chromatography[J].Journal of Agricultural and Food Chemistry, 1983,31(5):1047-1052.
[6] 孟康. 肉桂醛对香菇采后品质及膜脂代谢调控研究[D].南京:南京农业大学, 2021.
MENG K.Study on the regulation of cinnamaldehyde on postharvest quality and membrane lipid metabolism of Lentinula edodes[D].Nanjing:Nanjing Agricultural University, 2021.
[7] SHREAZ S, WANI W A, BEHBEHANI J M, et al.Cinnamaldehyde and its derivatives, a novel class of antifungal agents[J].Fitoterapia, 2016, 112:116-131.
[8] MALHEIRO J F, MAILLARD J Y, BORGES F, et al.Evaluation of cinnamaldehyde and cinnamic acid derivatives in microbial growth control[J].International Biodeterioration & Biodegradation,2019, 141:71-78.
[9] 何靖柳, 李玉, 杜小琴, 等.肉桂精油处理对低温贮藏期间‘红阳’猕猴桃抗氧化活性的影响[J].现代食品科技, 2016,32(3):211-217.
HE J L, LI Y, DU X Q, et al.Effects of cinnamon essential oil on the antioxidant activity of ‘red sun’ kiwifruits during cold storage[J].Modern Food Science and Technology, 2016,32(3):211-217.
[10] 万春鹏, 陈楚英, 陈明, 等.肉桂提取物对赣南脐橙的保鲜效果[J].食品工业科技, 2015,36(17):317-321.
WAN C P, CHEN C Y, CHEN M, et al.Effect of Cinnamomum cassia extractions on fresh-keeping of ‘Newhall’Navel orange[J].Science and Technology of Food Industry, 2015,36(17):317-321.
[11] GUO X G, CHEN B R, WU X L, et al.Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes[J].International Journal of Biological Macromolecules, 2020, 160:175-182.
[12] XIAO J Q, GU C Q, ZHU D X, et al.Development and characterization of an edible chitosan/zein-cinnamaldehyde nano-cellulose composite film and its effects on mango quality during storage[J].LWT, 2021, 140:110809.
[13] 张林青, 朱露露.大蒜乙醇提取液对生菜贮藏效果的影响[J].北方园艺, 2016(11):135-137.
ZHANG L Q, ZHU L L.Effect of garlic alcohol extract on lettuce storage[J].Northern Horticulture, 2016(11):135-137.
[14] 陈洲, 王婷婷, 姚颖斐, 等.黄连、连翘提取物对鲜切莴苣保鲜效果的研究[J].食品工业, 2013,34(11):180-183.
CHEN Z, WANG T T, YAO Y F, et al.Research on the preservation effect of Coptis chinensis and Forsythia suspensa on fresh-cut lettuce[J].The Food Industry, 2013, 34(11):180-183.
[15] 李铭桐, 张增芹, 李敏, 等.不同保鲜剂处理对鲜切生菜贮藏品质的影响[J].北方园艺, 2016(18):139-142.
LI M T, ZHANG Z Q, LI M, et al.Effect of different preservatives and storage temperature on the quality of fresh-cut lettuce[J].Northern Horticulture, 2016(18):139-142.
[16] KIM J H, KWON K H, OH S W.Effects of malic acid or/and grapefruit seed extract for the inactivation of common food pathogens on fresh-cut lettuce[J].Food Science and Biotechnology, 2016,25(6):1801-1804.
[17] 刘云芬, 廖玲燕, 段振华, 等.不同处理对鲜切莴苣褐变及贮藏品质的影响[J].保鲜与加工, 2020,20(4):15-20.
LIU Y F, LIAO L Y, DUAN Z H, et al.Effects of different treatments on browning and storage quality of fresh-cut lettuce[J].Storage and Process, 2020, 20(4):15-20.
[18] ZHOU F H, XU D Y, LIU C H, et al.Ascorbic acid treatment inhibits wound healing of fresh-cut potato strips by controlling phenylpropanoid metabolism[J].Postharvest Biology and Technology, 2021,181:111644.
[19] 曹建康, 姜微波, 赵玉梅.果蔬采后生理生化实验指导. 北京:中国轻工业出版社, 2007.
CAO J K, JIANG W B, ZHAO Y M. Guidance of Physiological and Biochemical Experiments on Postharvest Fruits and Vegetables. Beijing:China Light Industry Press, 2007.
[20] 李维嘉, 王志强, 许泽群, 等.分光光度法测定灵芝孢子油中总三萜的含量[J].食品研究与开发, 2019,49(17):165-170.
LI W J, WANG Z Q, XU Z Q, et al.Determination of total triterpenoids in Ganoderma lucidum spore oil by spectrophotometry[J].Food Research and Development, 2019, 49(17):165-170.
[21] LIU Y F, LIU Y, CHEN Q M, et al.Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage[J].Food Science & Nutrition, 2023,11(4):2049-2060.
[22] WANG D, CHEN Q Y, CHEN W W, et al.Melatonin treatment maintains quality and delays lignification in loquat fruit during cold storage[J].Scientia Horticulturae, 2021, 284:110126
[23] 谢晓宇, 张飞, 石洁, 等.预冷处理结合低温贮藏对西兰花贮藏品质的影响[J].食品工业科技, 2021,42(7):302-310.
XIE X Y, ZHANG F, SHI J, et al.Effect of pre-cooling treatment and low temperature storage on storage quality of broccoli[J].Science and Technology of Food Industry, 2021, 42(7):302-310.
[24] 李志晓, 金青哲, 叶小飞, 等.精炼过程中油茶籽油活性成分和抗氧化性的变化[J].中国油脂, 2015,40(8):1-5.
LI Z X, JIN Q Z, YE X F, et al.Changes of bioactive constituents and antioxidant activity of oil-tea Camellia seed oil during refining[J].China Oils and Fats, 2015, 40(8):1-5.
[25] LIN D S, SHUAI L, XU X L, et al.Salicylic acid inhibition on browning of fresh-cut taro by regulating phenolic compounds and active oxygen metabolism[J].LWT, 2024,206:116591.
[26] TILLEY A, MCHENRY M P, MCHENRY J A, et al.Enzymatic browning:The role of substrates in polyphenol oxidase mediated browning[J].Current Research in Food Science, 2023, 7:100623.
[27] CAI C, XU C J, LI X, et al.Accumulation of lignin in relation to change in activities of lignification enzymes in loquat fruit flesh after harvest[J].Postharvest Biology & Technology, 2006,40(2):163-169.
[28] TAN Y X, WANG X W, LI L, et al.Postharvest combined chitosan and melatonin treatment maintain antioxidant capacity and cell membrane integrity of fresh-cut broccoli by inducing reactive oxygen species scavenging system[J].LWT, 2025,220:117572.
[29] ALMUHAYAWI M S, HASSAN A H A, ABDEL-MAWGOUD M, et al.Laser light as a promising approach to improve the nutritional value, antioxidant capacity and anti-inflammatory activity of flavonoid-rich buckwheat sprouts[J].Food Chemistry, 2021,345:128788.
[30] 周晓斌. 控释型肉桂精油复合膜的制备及其在樱桃番茄保鲜中的应用研究[D].长春:吉林大学, 2024.
ZHOU X B.Preparation of controlled-release cinnamon essential oil composite films and their application in the preservation of cherry tomatoes[D].Changchun:Jilin University, 2024.
[31] SHUAI L, WANG M, LIAO L Y, et al.Trisodium phosphate inhibits the shrinkage of passion fruit by suppressing ROS accumulation and mitigating oxidative damage[J].LWT, 2025,218:117480.
[32] MEITHA K, PRAMESTI Y, SUHANDONO S.Reactive oxygen species and antioxidants in postharvest vegetables and fruits[J].International Journal of Food Science, 2020(1):8817778.
[33] XU D D, DENG Y Z, XI P G, et al.Fulvic acid-induced disease resistance to Botrytis cinerea in table grapes may be mediated by regulating phenylpropanoid metabolism[J].Food Chemistry, 2019, 286:226-233.
[34] ZHANG X B, LIU C J.Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids[J].Molecular Plant, 2015,8(1):17-27.
[35] 李静.切割方式和UV-C处理对鲜切莴苣品质及抗氧化活性的影响[D].南京:南京农业大学, 2017.
LI J.Effects of cutting methods and UV-C treatment on the quality and antioxidant activity of fresh-cut lettuce[D].Nanjing:Nanjing Agricultural University, 2017.
[36] 师聪, 刘大豪, 沈苏婉, 等.外源褪黑素处理对鲜切莴苣品质与活性氧代谢的影响[J].北方园艺, 2023(19):84-91.
SHI C, LIU D H, SHEN S W, et al.Effects of exogenous melatonin treatment on quality and reactive oxygen species metabolism of fresh-cut lettuce[J].Northern Horticulture, 2023(19):84-91.
[37] RU X Y, YOU W L, ZHANG J L, et al.γ-aminobutyric acid treatment inhibits browning and promotes storage quality by regulating reactive oxygen species and membrane lipid metabolism in fresh-cut stem lettuce[J].Food Chemistry, 2024,459:140420.
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