Food and Fermentation Industries >

2026 , Vol. 52 >Issue 8: 346 - 352

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043368

Functional evaluation and recombinant construction of lipopeptide surfactant from Sporosarcina koreensis

  • LIU Jialin ,
  • LI Meng ,
  • NING Xiao
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  • 1(College of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China)
    2(Key Laboratory of Food Quality and Safety for State Market Regulation, National Institute of Food and Drug Control, Beijing 100050, China)

Received date: 2025-05-27

  Revised date: 2025-08-28

  Online published: 2026-05-07

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Abstract

To support the application of non-model Bacillus strains in biosurfactant development, this study evaluated the functional properties of a laboratory-isolated strain WZ1 and performed preliminary genetic engineering to enhance its biosurfactant production.16S rDNA sequencing identified WZ1 as Sporosarcina koreensis.Fermentation, acid precipitation, and methanol extraction were used to obtain crude metabolites.Lipopeptide surfactants with antimicrobial activity were confirmed by oil-spreading assays, thin-layer chromatography (TLC), and HPLC.Antibacterial activity of the fermentation broth and crude extract was assessed via Oxford cup assays against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Aspergillus niger, showing consistent inhibition across three biological replicates.Regulatory genes ComX and PhrC were cloned and inserted into plasmid PHT 43.These constructs were introduced into WZ1, and enhanced surfactant production was evidenced by increased oil-spreading diameters.This work provides foundational insight into the functional expression of lipopeptide surfactants in S.koreensis and offers preliminary validation of a gene-regulatory strategy for improving biosurfactant yield in non-model Bacillus species.

Key words： surfactin; Sporosarcina koreensis; lipopeptide; oil displacement; antimicrobial activity; recombinant expression

Cite this article

LIU Jialin , LI Meng , NING Xiao . Functional evaluation and recombinant construction of lipopeptide surfactant from Sporosarcina koreensis[J]. Food and Fermentation Industries, 2026 , 52(8) : 346 -352 . DOI: 10.13995/j.cnki.11-1802/ts.043368

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