Research progress on extraction, determination, and biological activity of 1-deoxynojirimycin from mulberry leaves

  • WU Xiaohui ,
  • LIU Jielin ,
  • ZHOU Liangfu ,
  • ZHANG Yingying ,
  • CAO Jiacheng ,
  • LU Yaxin ,
  • TANG Xia ,
  • SHENG Qinghai
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  • 1(College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)
    2(Chengde Academy of Agriculture and Forestry Sciences, Chengde 067000, China)
    3(Hebei Provincial Key Laboratory of Dietary Component Interaction and Precision Nutrition, Baoding 071000, China)

Received date: 2025-07-09

  Revised date: 2025-08-21

  Online published: 2026-05-07

Abstract

1-deoxynojirimycin (DNJ) is a naturally occurring alkaloid that has been identified in mulberry leaves.It has been demonstrated that the substance under scrutiny is an effective glycosidase inhibitor, with the potential to reduce blood sugar levels.In addition to this, a number of biological activities have been observed in the substance, including the capacity to lower lipids, to act as an antiviral, and to elicit anticancer effects.The present paper sets out the various methodologies currently employed for the extraction of DNJ, including thermal extraction, ultrasonic extraction, microwave extraction and reflux extraction.The primary method for determining DNJ is high performance liquid chromatography.However, a comprehensive and methodical review of the extant literature on the biological activity of DNJ remains to be undertaken.This article comprehensively reviews the efficacy and mechanism of action of DNJ, and explores its extraction and determination methods, as well as the latest developments in research on its biological activity.The article's objective is to serve as a foundational reference for future research endeavours concerning DNJ.

Cite this article

WU Xiaohui , LIU Jielin , ZHOU Liangfu , ZHANG Yingying , CAO Jiacheng , LU Yaxin , TANG Xia , SHENG Qinghai . Research progress on extraction, determination, and biological activity of 1-deoxynojirimycin from mulberry leaves[J]. Food and Fermentation Industries, 2026 , 52(8) : 427 -435 . DOI: 10.13995/j.cnki.11-1802/ts.043773

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