Food and Fermentation Industries >

2026 , Vol. 52 >Issue 9: 1 - 9

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043897

Bioinformatic analysis of CRISPR/Cas system in Lactobacillus jensenii

  • HU Qi ,
  • LIAO Caiyu ,
  • TANG Jiachen ,
  • TANG Ke ,
  • CHENG Daomei ,
  • ZHAO Changsong ,
  • YU Meiyan ,
  • HAN Yunlei
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  • 1(School of Public Health, Chengdu Medical College, Chengdu 610500, China)
    2(School of Health and Intelligent Engineering, Chengdu Medical College, Chengdu 610500, China)
    3(School of Basic Medical Sciences, Chengdu Medical College, Chengdu 610500, China)

Received date: 2025-07-14

  Revised date: 2025-09-28

  Online published: 2026-06-03

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Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) and associated proteins (Cas) have been widely applied in genome editing and molecular diagnostics.To develop genome editing tools based on the Lactobacillus jensenii CRISPR/Cas system, this study performed a bioinformatic analysis of its structural features and potential mechanisms of action. A total of 177 L.jensenii genomes from the GenBank database were analyzed.Using CRISPRViz to identify CRISPR loci.CRISPROne predicted Cas protein types and tracrRNA positions.MEGA X was employed to construct phylogenetic trees.RNAfold analyzed the secondary structures of repeat sequences.CRISPRTarget performed spacer homology analysis and protospacer adjacent motif (PAM) prediction.Among the 177 genomes, 146 (82.49%) contained CRISPR loci, comprising 152 confirmed arrays.The CRISPR repeats ranged from 27 to 40 nucleotides, while spacer ranged from 28 to 40 nucleotides, with 3 to 44 spacers per array.A total of 144 strains carried cas9 genes, predominantly of partial subtype Ⅱ-A (128 strains, 88.89%), and subtype Ⅱ-A genes were detected in 15 strains (10.42%).One strain harbored cas genes from both subtypes.Additionally, 86 strains contained one tracrRNA gene, located in the non-coding region between cas1 and cas9 genes.Of the 769 unique spacers, 219 targeted phages, 59 targeted plasmids, and the remainder had unknown targets.The predicted PAM sequence recognized by L.jensenii Cas9 was 5′-CCA-3′.These findings provide a reference for developing CRISPR/Cas9-based gene editing tools for L.jensenii.

Key words： Lactobacillus jensenii; CRISPR/Cas; repeats; spacer; tracrRNA; protospacer adjacent motif

Cite this article

HU Qi , LIAO Caiyu , TANG Jiachen , TANG Ke , CHENG Daomei , ZHAO Changsong , YU Meiyan , HAN Yunlei . Bioinformatic analysis of CRISPR/Cas system in Lactobacillus jensenii[J]. Food and Fermentation Industries, 2026 , 52(9) : 1 -9 . DOI: 10.13995/j.cnki.11-1802/ts.043897

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