Food and Fermentation Industries >

2026 , Vol. 52 >Issue 10: 42 - 50

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043818

Antioxidant properties and human skin fibroblasts damage repair effects of solid-state fermentation mycelial extract of Stropharia rugosoannulata

  • XU Weitao ,
  • WU Di ,
  • LI Wen ,
  • CHEN Wanchao ,
  • LIU Peng ,
  • WU Jingting ,
  • ZHANG Zhong ,
  • WANG Guangqiang ,
  • YANG Yan
Expand
  • 1(School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)
    3(College of Life Sciences, Shanghai Normal University, Shanghai 200234, China)

Received date: 2025-07-08

  Revised date: 2025-10-17

  Online published: 2026-06-15

Fold

Abstract

The mycelium can produce a variety of bioactive metabolites in the solid-state fermentation process of Stropharia rugosoannulata, however, the potential applications of the resulting fermented biomass have seldom been investigated.This study aimed to explore the antioxidant capacity and cutaneous protective potential of the solid-state fermented biomass derived from S.rugosoannulata.Four extraction methods (aqueous extraction, ultrasound-assisted aqueous extraction, alkaline extraction, and ultrasound-assisted alkaline extraction) were employed to prepare the extracts.The active components of the extracts were analyzed, and the in vitro antioxidant activity was evaluated.Additionally, the protective effects of the extracts against ultraviolet B (UVB)-induced damage in human skin fibroblasts (HSFs) were assessed, along with their ability to promote mechanical scratch wound healing in HSFs.Results showed significant differences (P<0.05) in yield, protein, polysaccharide, polypeptide, and total phenolic content among extracts from different methods.Ultrasound-assisted alkali extraction had the highest yield (5.20%), but alkali extraction showed the best antioxidant capacity.In cell experiments, water extracts significantly protected UVB-damaged HSF cells in a dose-dependent manner.Further analysis found that the protein-rich fraction in water extracts SP2 had strong antioxidant capacity and provided more significant protection, with a cell viability of 89.05% at 125 μg/mL.This component also exhibited a significant effect on the scratch mechanical injury repair of HSF cells.In conclusion, the extracts from S.rugosoannulata solid-state fermentation biomass have antioxidant activity and skin-protective potential, offering a theoretical basis for their application in the field of cosmetics.

Key words： Stropharia rugosoannulata; solid-state fermentation biomass; antioxidant; human skin fibroblasts; skin protection

Cite this article

XU Weitao , WU Di , LI Wen , CHEN Wanchao , LIU Peng , WU Jingting , ZHANG Zhong , WANG Guangqiang , YANG Yan . Antioxidant properties and human skin fibroblasts damage repair effects of solid-state fermentation mycelial extract of Stropharia rugosoannulata[J]. Food and Fermentation Industries, 2026 , 52(10) : 42 -50 . DOI: 10.13995/j.cnki.11-1802/ts.043818

References

[1] 王米雪, 圣志存, 陈晓兰.大球盖菇发酵菌丝体多糖提取条件优化与结构分析[J].北方园艺, 2020(1):111-116.
WANG M X, SHENG Z C, CHEN X L.Optimization of extraction and characterization of polysaccharide from Stropharia rugosoannulata fermentation mycelium[J].Northern Horticulture, 2020(1):111-116.
[2] 安志刚, 孙逸来, 张振国, 等.四种食药菌固体发酵菌质活性物质及其抗氧化能力研究[J].中央民族大学学报(自然科学版), 2023, 32(4):36-43.
AN Z G, SUN Y L, ZHANG Z G, et al.Active substances and antioxidant capacity of solid fermentation substance by four edible and medicinal fungi[J].Journal of Minzu University of China (Natural Sciences Edition), 2023, 32(4):36-43.
[3] 李奕葶, 翁正杭, 黄亮, 等.利用香菇菌丝体发酵藜麦及其发酵产物中粗多糖的抗氧化评价[J].食品科技, 2020, 45(3):18-22.
LI Y T, WENG Z H, HUANG L, et al.The antioxidant evaluation of crude polysacharide from fermented quinoa by Lentinus edodes[J].Food Science and Technology, 2020, 45(3):18-22.
[4] LI Y T, BAN L T, MENG S L, et al.Bioactivities of crude polysaccharide extracted from fermented soybean curd residue by Cordyceps militaris[J].Biotechnology & Biotechnological Equipment, 2021, 35(1):334-345.
[5] 郝海波, 岳一鸿, 王倩, 等.大球盖菇新品种‘申球2号’[J].园艺学报, 2025, 52(S1):137-138.
HAO H B, YUE Y H, WANG Q, et al.A new Stropharia rugosoannulata cultivar ‘Shenqiu 2’[J].Acta Horticulturae Sinica, 2025, 52(S1):137-138.
[6] 高琛, 李文, 陈万超, 等.3种食用菌蛋白质-多糖复合物的体内外降血脂作用[J].食品科学, 2024, 45(24):117-127.
GAO C, LI W, CHEN W C, et al.In vitro and in vivo hypolipidemic effects of protein-polysaccharide complexes from three species of edible fungi[J].Food Science, 2024, 45(24):117-127.
[7] 张珣, 王婧, 丁华, 等.DNS法测定茶叶中掺杂蔗糖含量[J].湖北农业科学, 2021, 60(21):107-111.
ZHANG X, WANG J, DING H, et al.Study on the DNS method for measuring the content of doped sucrose in tea[J].Hubei Agricultural Sciences, 2021, 60(21):107-111.
[8] 邵哲, 喻治达, 钟袁源.红托竹荪菌托黄酮的纯化及其抗氧化与抗疲劳活性研究[J].食品安全质量检测学报, 2023, 14(1):315-322.
SHAO Z, YU Z D, ZHONG Y Y.Study on purification, anti-oxidation and anti-fatigue activity of flavonoids from Dictyophora rubrovolvata[J].Journal of Food Safety & Quality, 2023, 14(1):315-322.
[9] SHI M, ZHANG Z Y, YANG Y N.Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide (GLP)[J].Carbohydrate Polymers, 2013, 95(1):200-206.
[10] LI W, JI J, CHEN X H, et al.Structural elucidation and antioxidant activities of exopolysaccharides from Lactobacillus helveticus MB2-1[J].Carbohydrate Polymers, 2014, 102:351-359.
[11] LIN C H, WEI Y T, CHOU C C.Enhanced antioxidative activity of soybean koji prepared with various filamentous fungi[J].Food Microbiology, 2006, 23(7):628-633.
[12] 李紫薇. 红托竹荪栽培废弃菌托胶质的分离制备及应用于护肤品的活性探究[D].上海:上海师范大学, 2025.
LI Z W.Isolation and preparation of mucilage from the cultivation waste (volva) of Dictyophora rubrovolvata and investigation of its bioactivity for cosmetic applications[D].Shanghai:Shanghai Normal University, 2025.
[13] GADALKAR S M, RATHOD V K.Extraction of watermelon seed proteins with enhanced functional properties using ultrasound[J].Preparative Biochemistry & Biotechnology, 2020, 50(2):133-140.
[14] 赵芷芊, 王敏, 张志清.植物多糖的提取及抗氧化功效的研究进展[J].食品工业科技, 2018, 39(13):337-342.
ZHAO Z Q, WANG M, ZHANG Z Q.Research progress of antioxidation efficacy and extraction of plant polysaccharide[J].Science and Technology of Food Industry, 2018, 39(13):337-342.
[15] 王 帅,钟明明,王震霄, 等.超声辅助pH值偏移处理对大豆亲脂蛋白结构和功能性质的影响及其相关性分析[J].食品科学, 2024,45 (16):169-176.
WANG S, ZHONG M M,WANG Z X, et al.Effect of ultrasound-assisted pH shift on the structural and functional properties of soybean lipophilic proteins and correlation between them[J].Food Science, 2024,45 (16):169-176.
[16] 朱少娟, 施用晖, 乐国伟.超声波对胰蛋白酶水解酪蛋白的影响[J].食品与生物技术学报, 2005, 24(2):50-54.
ZHU S J, SHI Y H, YUE G W.Effects of ultrasound on the hydrolysis of casein by trypsin[J].Journal of Food Science and Biotechnology, 2005, 24(2):50-54.
[17] 孙玉姣, 马芸皓, 王凡, 等.不同提取方法对茯茶多糖理化性质和抗氧化作用的影响[J].陕西科技大学学报, 2021, 39(5):31-38.
SUN Y J, MA Y H, WANG F, et al.Influence of different extraction methods on physicochemical properties and antioxidant effects of Fu Brick tea polysaccharides[J].Journal of Shaanxi University of Science & Technology, 2021, 39(5):31-38.
[18] OJEDA G A, SGROPPO S C, SÁNCHEZ-MORENO C, et al.Mango ‘criollo’ by-products as a source of polyphenols with antioxidant capacity.Ultrasound assisted extraction evaluated by response surface methodology and HPLC-ESI-QTOF-MS/MS characterization[J].Food Chemistry, 2022, 396:133738.
[19] MORSHED M N, AHN J C, MATHIYALAGAN R, et al.Antioxidant activity of Panax ginseng to regulate ROS in various chronic diseases[J].Applied Sciences, 2023, 13(5):2893.
[20] 陈湘宁, 张艳艳, 李里特, 等.大豆蛋白水解产物中巯基的热稳定性和抗氧化作用的研究[J].食品与发酵工业, 2005,31(7):19-22.
CHEN X N, ZHANG Y Y, LI L T, et al.Heat-stability and antioxidative activity of sulfhydryls in soy protein hydrolysate[J].Food and Fermentation Industries, 2005,31(7):19-22.
[21] LIU X Y, XING Y, YUEN M, et al.Anti-aging effect and mechanism of proanthocyanidins extracted from Sea buckthorn on hydrogen peroxide-induced aging human skin fibroblasts[J].Antioxidants, 2022,11(10):1900.
[22] 刘爽, 刘爱青, 王佳, 等.不同胶原蛋白肽对光损伤皮肤细胞的修复作用[J].食品工业, 2023,44(10):125-130.
LIU S, LIU A Q, WANG J, et al.Effects of different collagen peptide and collagen tripeptide on photoaging in UVB-exposed human skin fibroblasts[J].The Food Industry, 2023,44(10):125-130.
[23] 沙金, 杨华, 庞敏霞, 等.醇沉浓度对铁皮石斛茎、叶和花多糖结构及其特性的影响[J].核农学报, 2025, 39(6):1235-1247.
SHA J, YANG H, PANG M X, et al.Effects of alcohol precipitation with different concentrations on the structure and properties of polysaccharides from stems, leaves and flowers of Dendrobium officinale[J].Journal of Nuclear Agricultural Sciences, 2025, 39(6):1235-1247.
[24] 赵小莉, 付洋, 赵强, 等.硫酸铵沉淀分离富硒大豆蛋白的初步研究[J].食品工业科技, 2016, 37(13):92-95;100.
ZHAO X L, FU Y, ZHAO Q, et al.Preliminary study on ammonium sulfate precipitation of selenium-enriched soybean protein isolate[J].Science and Technology of Food Industry, 2016, 37(13):92-95;100.
[25] GÓMEZ-GUILLÉN M C, GIMÉNEZ B, LÓPEZ-CABALLERO M E, et al.Functional and bioactive properties of collagen and gelatin from alternative sources:A review[J].Food Hydrocolloids, 2011, 25(8):1813-1827.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.