In this study, a hyperuricemic (HUA) mice model was established to investigate the synergistic mechanism of the combined intervention of Lactiplantibacillus plantarum KLDS 1.0328 and puerarin in hyperuricemia.The study revealed that compared with the HUA group, the combination of L.plantarum and puerarin significantly reduced the levels of uric acid to (38.81±6.78) μmol/L.The combination of L.plantarum and puerarin suppressed serum and liver xanthine oxidase activity, improved renal function, and reduced kidney damage.Moreover, L.plantarum combined with puerarin could regulate the expression of colonic tight junction proteins (Occludin, Claudin-1, ZO-1), thereby repairing the intestinal barrier.Additionally, the combination inhibited the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), while elevating the levels of anti-inflammatory cytokine IL-10.The intervention enhanced glutathione peroxidase and superoxide dismutase activity but reduced malondialdehyde levels and myeloperoxidase activity, mitigating renal and colonic inflammation and oxidative stress.Furthermore, the combination of L.plantarum and puerarin suppressed the expression of crucial proteins within NLRP3/NF-κB pathways and NLRP3 inflammasome.The intervention of L.plantarum and puerarin restored the diversity of gut microbiota in mice with hyperuricemia and regulated the structure of gut microbiota.Accordingly, these findings demonstrated that co-intervention with L. plantarum KLDS1.0328 and puerarin effectively alleviates HUA, providing a theoretical foundation and empirical evidence for the development of functional foods targeting uric acid metabolism regulation.
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