Food and Fermentation Industries >

2026 , Vol. 52 >Issue 10: 175 - 181

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044148

Ultra-high performance liquid chromatography coupled with Q-exactive orbitrap tandem mass spectrometry analysis of chemical constituents and quality markers in Sanghuangporus sanghuang

  • GUO Xinru ,
  • GAO Jinming ,
  • LIU Shuhui
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  • (College of Chemistry and Pharmacy, Northwest A&F University, Yangling 712100, China)

Received date: 2025-08-11

  Revised date: 2025-10-19

  Online published: 2026-06-15

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Abstract

Sanghuangporus sanghuang is a medicinal macrofungus that has long been utilized in East Asia for its diverse pharmacological properties, including antioxidant, anti-inflammatory, and anticancer activities.Despite its increasing use, the chemical basis underlying these bioactivities remains incompletely characterized, and systematic comparisons between cultivated and wild resources are scarce.This study comprehensively characterized the chemical constituents of both cultivated and wild S.sanghuang and identified the potential quality markers using ultra-high performance liquid chromatography coupled with Q-exactive orbitrap tandem mass spectrometry.The enrichment factors were calculated based on peak area ratios between wild and cultivated samples.A total of forty-six chemical constituents were unambiguously identified.Among them, six flavonoids, six isocoumarins, nine phenolics, and two phenylpropanoids were enriched in wild specimens, with enrichment factors ranging from 0.026 to 0.610.Furthermore, ten out of eighteen styrylpyrones exhibited preferential accumulation in wild samples (0.035-0.332).Conversely, cultivated specimens contained markedly higher levels of ergosterol, three of the four fatty acids, and three styrylpyrones, with enrichment factors spanning 2.724 to 5.432.On the basis of differential distribution patterns and bioactivity relevance, Hispidin, Inoscavin A, Hypholomine B, Phelligridin A, and ergosterol were proposed as quality markers for S.sanghuang.These findings provide a scientific foundation for the standardization of S.sanghuang cultivation and the development of evidence-based quality control frameworks.

Key words： Sanghuangporus sanghuang; chemical constituents; content differences; quality marker; ultra-high performance liquid chromatography coupled with Q-exactive orbitrap tandem mass spectrometry analysis

Cite this article

GUO Xinru , GAO Jinming , LIU Shuhui . Ultra-high performance liquid chromatography coupled with Q-exactive orbitrap tandem mass spectrometry analysis of chemical constituents and quality markers in Sanghuangporus sanghuang[J]. Food and Fermentation Industries, 2026 , 52(10) : 175 -181 . DOI: 10.13995/j.cnki.11-1802/ts.044148

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