Research progress on immunoassay methods for common pathogenic Vibrio species

  • HUANG Mingyue ,
  • HAN Lu ,
  • GONG Fang ,
  • LI Sijia ,
  • YU Xiang ,
  • WANG Kaile ,
  • CHEN Xiujin ,
  • LI Zhaozhou ,
  • WANG Yao ,
  • KANG Huaibin
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  • 1(College of Food and Bioengineering, Henan Key Laboratory of Biomarker Rapid Detection Technology for Food and Drug Safety, Henan University of Science and Technology, Luoyang 471023, China)
    2(Henan Anzhiyuan Detection Technology Co.Ltd., Kaifeng 475500, China)

Received date: 2025-09-06

  Revised date: 2025-10-29

  Online published: 2026-07-17

Abstract

Pathogenic Vibrio species are common foodborne pathogens in aquatic products and are a leading cause of food poisoning.Rapid, sensitive, and accurate detection methods are crucial for monitoring pathogenic Vibrio and preventing foodborne diseases.Immunoassay methods offer significant advantages in sensitivity, specificity, field applicability, and simplified sample preparation, making them a focal point in pathogenic Vibrio detection research.This review begins by summarizing the types of antibodies against pathogenic Vibrio and their preparation methods, and then examines the current applications of techniques such as enzyme-linked immunosorbent assay, immunofluorescence, immunochromatography, and immunomagnetic bead separation.It aims to provide references for the efficient monitoring and effective prevention of pathogenic Vibrio.

Cite this article

HUANG Mingyue , HAN Lu , GONG Fang , LI Sijia , YU Xiang , WANG Kaile , CHEN Xiujin , LI Zhaozhou , WANG Yao , KANG Huaibin . Research progress on immunoassay methods for common pathogenic Vibrio species[J]. Food and Fermentation Industries, 2026 , 52(12) : 436 -445 . DOI: 10.13995/j.cnki.11-1802/ts.044445

References

[1] LAVERTY A L, PRIMPKE S, LORENZ C, et al.Bacterial biofilms colonizing plastics in estuarine waters, with an emphasis on Vibrio spp.and their antibacterial resistance[J].PLoS One, 2020, 15(8):e0237704.
[2] FROELICH B A, DAINES D A.In hot water:Effects of climate change on Vibrio-human interactions[J].Environmental Microbiology, 2020, 22(10):4101-4111.
[3] BAKER-AUSTIN C, OLIVER J D, ALAM M, et al.Vibrio spp. infections[J].Nature Reviews Disease Primers, 2018, 4(1):5.
[4] ROBERT-PILLOT A, COPIN S, HIMBER C, et al.Occurrence of the three major Vibrio species pathogenic for human in seafood products consumed in France using real-time PCR[J].International Journal of Food Microbiology, 2014, 189:75-81.
[5] BONNIN-JUSSERAND M, COPIN S, LE BRIS C, et al.Vibrio species involved in seafood-borne outbreaks (Vibrio cholerae, V.parahaemolyticus and V-vulnificus):Review of microbiological versus recent molecular detection methods in seafood products[J].Critical Reviews in Food Science and Nutrition, 2019, 59(4):597-610.
[6] YIN J F, WANG M Y, CHEN Y J, et al.Direct detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio alginolyticus from clinical and environmental samples by a multiplex touchdown polymerase chain reaction assay[J].Surgical Infections, 2018, 19(1):48-53.
[7] LENG F, LIN S L, WU W, et al.Epidemiology, pathogenetic mechanism, clinical characteristics, and treatment of Vibrio vulnificus infection:A case report and literature review[J].European Journal of Clinical Microbiology & Infectious Diseases, 2019, 38(11):1999-2004.
[8] MOK J S, RYU A, KWON J Y, et al.Abundance, antimicrobial resistance, and virulence of pathogenic Vibrio strains from molluscan shellfish farms along the Korean coast[J].Marine Pollution Bulletin, 2019, 149:110559.
[9] WU W, JING Z H, YU X Q, et al.Recent advances in screening aquatic products for Vibrio spp[J].TrAC Trends in Analytical Chemistry, 2019, 111:239-251.
[10] VEZZULLI L, COLWELL R R, PRUZZO C.Ocean warming and spread of pathogenic Vibrios in the aquatic environment[J].Microbial Ecology, 2013, 65(4):817-825.
[11] BRAUGE T, MOUGIN J, ELLS T, et al.Sources and contamination routes of seafood with human pathogenic Vibrio spp.:A Farm-to-Fork approach[J].Comprehensive Reviews in Food Science and Food Safety, 2024, 23(1):e13283.
[12] TOROK V A, MAHBUB K R, GREY P A, et al.Survey of foodborne pathogenic Vibrio species in commercial Tasmanian bivalve shellfish and predictors of risk at harvest[J].International Journal of Food Microbiology, 2025, 430:111033.
[13] 谢雪钦, 刘舟.免疫学方法在食源性副溶血性弧菌检测中的应用研究进展[J].食品科学, 2017, 38(13):299-305.
XIE X Q, LIU Z.Recent advances in application of immunological methods in detection of foodborne Vibiro parahaemolyticus[J].Food Science, 2017, 38(13):299-305.
[14] 朱慧, 李嘉文, 绳秀珍, 等.副溶血弧菌胶体金快速检测试纸的研制及应用[J].中国海洋大学学报(自然科学版), 2021, 51(3):24-33.
ZHU H, LI J W, SHENG X Z, et al.Development of a competitive gold immunochromatographic test strip for rapid detection of Vibrio parahaemolyticus in fish[J].Periodical of Ocean University of China, 2021, 51(3):24-33.
[15] PATTIPEILOHY M, SUNDARI S.The immunogenicity of polyclonal antibody from induced Vibrio parahaemolyticus membrane by Elisa method[J].Journal of Physics:Conference Series, 2019, 1146(1):012032
[16] BAYAT M, KHABIRI A, HEMATI B.Development of IgY-based sandwich ELISA as a robust tool for rapid detection and discrimination of toxigenic Vibrio cholerae[J].Canadian Journal of Infectious Diseases & Medical Microbiology, 2018, 2018:4032531.
[17] WANG M Y, LIU X F, XIA J, et al.Vibrio vulnificus VvhA induces Th1 and Tfh cells to proliferate against Vibrio vulnificus in a mouse model of infection[J].Future Microbiology, 2017, 12:953-965.
[18] SITHIGORNGUL W, RENGPIPAT S, TANSIRISITTIKUL A, et al.Development of monoclonal antibodies for simple identification of Vibrio alginolyticus[J].Letters in Applied Microbiology, 2006, 43(4):436-442.
[19] MARTIN S J, SIEBELING R J.Identification of Vibrio vulnificus O serovars with antilipopolysaccharide monoclonal antibody[J].Journal of Clinical Microbiology, 1991, 29(8):1684-1688.
[20] WANGMAN P, CHAIVISUTHANGKURA P, TAENGCHAIYAPHUM S, et al.Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease[J].Journal of Fish Diseases, 2020, 43(2):207-214.
[21] ZHANG H Y, WANG Q, ZHUANG H X, et al.Development of recombinant antibodies and its application in immunomagnetic separation-based rapid detection of Vibrio cholerae in aquatic environments[J].Journal of Microbiology and Biotechnology, 2024, 34(11):2266-2278.
[22] 潘振, 陈杰敏, 胡成进.创伤弧菌溶细胞素噬菌体单链抗体库的构建[J].中华卫生应急电子杂志, 2021, 7(5):287-291.
PAN Z, CHEN J M, HU C J.Construction of mouse-derived phage display library of scFv fragments against Vibrio vulnificus VvhA[J].Chinese Journal of Hygiene Rescue (Electronic Edition), 2021, 7(5):287-291.
[23] WANG Y Q, ZHANG L, WANG P, et al.Enhancing oriented immobilization efficiency:A one-for-two organism-bispecific nanobody scaffold for highly sensitive detection of foodborne pathogens[J].Analytical Chemistry, 2023, 95(46):17135-17142.
[24] SHAO S R, YANG H R, ZENG Q F, et al.Generation and evaluation of nanobodies specific for pirA and pirB from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease[J].Aquaculture, 2025, 602:742357.
[25] EBRAHIMI MOJARAD A, MOUSAVI GARGARIA S L.Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1[J].Iranian Journal of Microbiology, 2020, 12(4):263-272.
[26] 陈奇. 单核细胞增生性李斯特菌单克隆抗体、多克隆抗体及单域重链抗体的制备[D].南昌:南昌大学, 2014.
CHEN Q.Preparation of anti-Listeria monocytogenes monoclonal antibody, polyclonal antibodies and single-domain heavy chain antibody[D].Nanchang:Nanchang University, 2014.
[27] NAKAZAWA M, MUKUMOTO M, MIYATAKE K.Production and Purification of Polyclonal Antibodies[M].High-Resolution Imaging of Cellular Proteins:Methods and Protocols.New York, NY:Springer New York.2016:49-59.
[28] 李世佳. 利用CRISPR技术敲除兔细胞HGPRT基因[D].福州:福州大学, 2018.
LI S J.Knockout of HGPRT in rabbit cell by CRISPR/Cas9 technique[D].Fuzhou:Fuzhou University, 2018.
[29] QRIOUET Z, CHERRAH Y, SEFRIOUI H, et al.Monoclonal antibodies application in lateral flow immunochromatographic assays for drugs of abuse detection[J].Molecules, 2021, 26(4):1058
[30] 全美淋, 李飞, 刘金燕, 等.抗体技术在食源性致病菌快速检测中的研究进展[J].生物工程学报, 2024, 40(11):3985-4005.
QUAN M L, LI F, LIU J Y, et al.Research progress of antibody technology in rapid detection of foodborne pathogens[J].Chinese Journal of Biotechnology, 2024, 40(11):3985-4005.
[31] HOLLIGER P, HUDSON P J.Engineered antibody fragments and the rise of single domains[J].Nature Biotechnology, 2005, 23(9):1126-1136.
[32] ZHAO Q, LU D, ZHANG G Y, et al.Recent improvements in enzyme-linked immunosorbent assays based on nanomaterials[J].Talanta, 2021, 223:121722.
[33] 肖益群, 翟少伟, 郭松林.鳗鲡源创伤弧菌的间接ELISA快速检测[J].集美大学学报(自然科学版), 2020, 25(2):81-87.
XIAO Y Q, ZHAI S W, GUO S L.The indirect ELISA for rapid detection of Vibrio vulnificus from eels[J].Journal of Jimei University (Natural Science), 2020, 25(2):81-87.
[34] 曾静, 程晋霞, 张蕾, 等.创伤弧菌鞭毛蛋白单克隆抗体的制备及在食品检测中的应用[J].细胞与分子免疫学杂志, 2013, 29(10):1068-1071;1075.
ZENG J, CHENG J X, ZHANG L, et al.Preparation of monoclonal antibodies against flagellin core protein of Vibrio vulnificus and its application in testing Vibrio vulnificus from food products[J].Chinese Journal of Cellular and Molecular Immunology, 2013, 29(10):1068-1071;1075.
[35] 职通瑞, 宋晓玲, 张晓静, 等.多抗间接ELISA方法的建立及其在海洋细菌快速检测中的应用[J].渔业科学进展, 2015, 36(2):71-76.
ZHI T R, SONG X L, ZHANG X J, et al.Establishment of polyclonal antibody indirect enzyme-linked immunosorbent assay (ELISA) and the application in the rapid detection of marine bacteria[J].Progress in Fishery Sciences, 2015, 36(2):71-76.
[36] 程晋霞, 曾静, 张蕾, 等.霍乱弧菌鞭毛蛋白单克隆抗体的制备及双抗体夹心ELISA的建立[J].细胞与分子免疫学杂志, 2013, 29(11):1169-1173.
CHENG J X, ZENG J, ZHANG L, et al.Preparation of monoclonal antibodies against flagellin core protein of Vibrio cholerae and its application in establishing double-antibody sandwich ELISA for testing Vibrio cholerae from food products[J].Chinese Journal of Cellular and Molecular Immunology, 2013, 29(11):1169-1173.
[37] HU Y Z, SUN Y, GU J X, et al.Selection of specific nanobodies to develop an immuno-assay detecting Staphylococcus aureus in milk[J].Food Chemistry, 2021, 353:129481.
[38] ZHANG C, LIU Z L, BAI M F, et al.An ultrasensitive sandwich chemiluminescent enzyme immunoassay based on phage-mediated double-nanobody for detection of Salmonella Typhimurium in food[J].Sensors and Actuators B:Chemical, 2022, 352:131058.
[39] WEI T X, DU D, ZHU M J, et al.An improved ultrasensitive enzyme-linked immunosorbent assay using hydrangea-like antibody-enzyme-inorganic three-in-one nanocomposites[J].ACS Applied Materials & Interfaces, 2016, 8(10):6329-6335.
[40] MONDAL S.Immunofluorescence Techniques[M].Manual of Histological Techniques, 2018.
[41] 王金雨, 刘志超, 赵晓东. 免疫荧光技术及其在水产动物疾病检测中的应用[J]. 江西水产科技, 2017(2): 37-39.
WANG J Y, LIU Z C, ZHAO X D. Immunofluorescence technique and its application in disease detection of aquatic animals[J]. Jiangxi Fishery Science and Technology, 2017(2): 37-39.
[42] 于光, 陈贵连, 王文. 吖啶橙免疫荧光菌团法快速检测鱼贝类副溶血性弧菌的研究[J]. 兽医大学学报, 1991, 11(4): 365-368.
YU G, CHEN G L, WANG W. Rapid detection of Vibrio parahaemoiyticus in sellfish by acridine orange immunofluorescent bacterial clumps method[J]. Chinese Journal of Veterinary Science, 1991, 11(4): 365-368.
[43] CHEN H C, CHANG T C.Rapid detection of Vibrio parahaemolyticus in oysters by immunofluorescence microscopy[J].International Journal of Food Microbiology, 1996, 29(2-3):311-319.
[44] WANG D C, XU X B, DENG X L, et al.Detection of Vibrio cholerae O1 and O139 in environmental water samples by an immunofluorescent-aggregation assay[J].Applied and Environmental Microbiology, 2010, 76(16):5520-5525.
[45] 王殿夫, 丁宁, 刘洪伟, 等.致病性弧菌检测新技术研究进展[J].食品安全质量检测学报, 2024, 15(2):160-167.
WANG D F, DING N, LIU H W, et al.Research progress in new technologies for detecting pathogenic Vibrio[J].Journal of Food Safety & Quality, 2024, 15(2):160-167.
[46] 张文红. 高灵敏度免疫荧光技术在动物免疫抗体检测中的精准性与可行性研究[J].智慧农业导刊, 2024, 4(7):39-42.
ZHANG W H.Research on accuracy and feasibility of high-sensitivity immunofluorescence technique in animal immune antibody detection[J].Journal of Smart Agriculture, 2024, 4(7):39-42.
[47] GONDHALEKAR C, BIELA E, RAJWA B, et al.Detection of E.coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy[J].Analytical and Bioanalytical Chemistry, 2020, 412(6):1291-1301.
[48] FANG J H, LI S Y, LIU X X, et al.Development of a test strip for rapid detection of Gymnodinium catenatum[J].Environmental Monitoring and Assessment, 2022, 195(1):83.
[49] DONG X, XIE J M, WANG L Y, et al.Development of monoclonal antibody against PirB and establishment of a colloidal gold immunochromatographic assay for the rapid detection of AHPND-causing Vibrio[J].Animals, 2024, 14(11):1600.
[50] 吴美娇, 吴有雪, 刘程, 等.基于组合胶体金纳米颗粒的免疫层析试纸条快速可视化检测海产品中副溶血性弧菌[J].工业微生物, 2021, 51(2):1-9.
WU M J, WU Y X, LIU C, et al.Rapid visual detection of Vibrio parahaemolyticus in seafood by immunochromatographic strip based on composited colloidal gold nanoparticles[J].Industrial Microbiology, 2021, 51(2):1-9.
[51] SAKATA J, YONEKITA T, KAWATSU K.Development of a rapid immunochromatographic assay to detect contamination of raw oysters with enteropathogenic Vibrio parahaemolyticus[J].International Journal of Food Microbiology, 2018, 264:16-24.
[52] DING C C, LI J W, LIU X, et al.Development of colloidal gold-based immunochromatographic strip test using two monoclonal antibodies for detection of Vibrio parahaemolyticus[J].Journal of Food Safety, 2018, 38(4):e12468.
[53] CHEN X R, LENG Y K, HAO L W, et al.Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format[J].Theranostics, 2020, 10(8):3737-3748.
[54] 李霁薇. 高灵敏检测两种食源性致病菌的聚集诱导发光免疫层析试纸条的开发[D].南昌:南昌大学, 2024.
LI J W.Development of highly sensitive immunochromatographic test strips based on aggregation-induced emission for the detection of two foodborne pathogen[D].Nanchang:Nanchang University, 2024.
[55] CHEN S P, JI L, XIE J P, et al.Silica-coated CdTe nanomaterial as high-affinity red fluorescence signal label for the detection of Vibrio vulnificus by lateral flow immunoassay[J].Food Control, 2025, 172:111190.
[56] BHARDWAJ N, BHARDWAJ S K, BHATT D, et al.Highly sensitive optical biosensing of Staphylococcus aureus with an antibody/metal-organic framework bioconjugate[J].Analytical Methods, 2019, 11(7):917-923.
[57] 苏娜, 明亮, 何静, 等.免疫检测技术在食源性致病菌中的应用研究进展[J].食品与发酵工业, 2024, 50(12):343-352.
SU N, MING L, HE J, et al.Research progress on application of immunoassays in foodborne pathogens[J].Food and Fermentation Industries, 2024, 50(12):343-352.
[58] MAO Y, HUANG X L, XIONG S C, et al.Large-volume immunomagnetic separation combined with multiplex PCR assay for simultaneous detection of Listeria monocytogenes and Listeria ivanovii in lettuce[J].Food Control, 2016, 59:601-608.
[59] LIU Y Y, ZHANG Z H, WANG Y L, et al.A highly sensitive and flexible magnetic nanoprobe labeled immunochromatographic assay platform for pathogen Vibrio parahaemolyticus[J].International Journal of Food Microbiology, 2015, 211:109-116.
[60] ZHAO L C, LYU X R, CAO X, et al.Improved quantitative detection of VBNC Vibrio parahaemolyticus using immunomagnetic separation and PMAxx-qPCR[J].Food Control, 2020, 110:106962.
[61] ZHAI Y, MENG X J, LI L, et al.Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence[J].RSC Advances, 2021, 11(61):38638-38647.
[62] LUN J S, LIU D, LIU T K, et al.Evaluation of outer membrane protein U (OmpU) as a novel capture target of Vibrio parahaemolyticus and rapid detection of acute hepatopancreatic necrosis disease (AHPND) using PCR combined with immunomagnetic separation[J].Aquaculture, 2018, 485:225-232.
[63] 郭姜里, 杜娟, 李宗双, 等.免疫磁分离技术在食源性致病菌前处理中的应用进展[J].食品安全质量检测学报, 2023, 14(21):97-106.
GUO J L, DU J, LI Z S, et al.Application of immunomagnetic separation technology in pretreatment of foodborne pathogenic bacteria[J].Journal of Food Safety & Quality, 2023, 14(21):97-106.
[64] 曹潇, 赵力超, 陈洵, 等.免疫磁分离技术在食源性致病菌快速检测中的研究进展[J].食品科学, 2019, 40(15):338-345.
CAO X, ZHAO L C, CHEN X, et al.Progress in the application of immunomagnetic separation in rapid detection of foodborne pathogens[J].Food Science, 2019, 40(15):338-345.
[65] 林吉恒, 黄朱梁, 彭志兰, 等.免疫磁珠分离技术在食源性致病菌检测中的应用[J].食品安全质量检测学报, 2019, 10(18):5998-6005.
LIN J H, HUANG Z L, PENG Z L, et al.Application of immunomagnetic beads separation techniques in detection of foodborne pathogenic bacteria[J].Journal of Food Safety & Quality, 2019, 10(18):5998-6005.
[66] HUANG H, LIU M H, WANG X S, et al.Label-free 3D Ag nanoflower-based electrochemical immunosensor for the detection of Escherichia coli O157:H7 pathogens[J].Nanoscale Research Letters, 2016, 11(1):507.
[67] YU J, STANKOVIC D, VIDIC J, et al.Recent advances in electrochemiluminescence immunosensing[J].Sensors & Diagnostics, 2024, 3(12):1887-1898.
[68] 杨兴东, 曲扬, 胡骁飞, 等.免疫分析方法在氧氟沙星残留检测中的研究进展[J].浙江农业科学, 2025, 66(3):742-749.
YANG X D, QU Y, HU X F, et al.Research progress of immunoassay methods in the detection of ofloxacin residues[J].Journal of Zhejiang Agricultural Sciences, 2025, 66(3):742-749.
[69] 郝寿辰. 快速检测副溶血性弧菌光电化学免疫传感器的构建及研究[D].大连:大连工业大学, 2019.
HAO S C.Construction and study of photoelectrochemical immunosensor for rapid detection of Vibrio parahaemolyticus[D].Dalian:Dalian University of Technology, 2019.
[70] SONG X X, XU L H, WU L, et al.Electrochemical immunosensor for detection of Vibrio parahaemolyticus based on faradaycage-type anodic stripping voltammetry[J].Journal of the Electrochemical Society, 2017, 164(13):B704-B709.
[71] MAHHEIDARI N, RASHIDIANI J, KOOSHKI H, et al.An effort to making a colorimitric nano-biosensor for Vibrio cholera detection[J].Current Nanoscience, 2020, 16(5):793-804.
[72] KOURTI D, ANGELOPOULOU M, PETROU P, et al.Optical immunosensors for bacteria detection in food matrices[J].Chemosensors, 2023, 11(8):430.
[73] YANG Y, LI G L, WANG P X, et al.Highly sensitive multiplex detection of foodborne pathogens using a SERS immunosensor combined with novel covalent organic frameworks based biologic interference-free Raman tags[J].Talanta, 2022, 243:123369.
[74] WU W, ZHOU M, HE H, et al.A sensitive aptasensor for the detection of Vibrio parahaemolyticus[J].Sensors and Actuators B:Chemical, 2018, 272:550-558.
[75] WANG P, YU G G, WEI J, et al.A single thiolated-phage displayed nanobody-based biosensor for label-free detection of foodborne pathogen[J].Journal of Hazardous Materials, 2023, 443:130157.
[76] ZHANG D Y, CHEN L, LIN H, et al.Well plate-based LF-NMR/colorimetric dual-mode homogeneous immunosensor for Vibrio parahaemolyticus detection[J].Food Chemistry, 2024, 436:137757.
[77] 王鹏. 副溶血性弧菌纳米抗体的研制及免疫分析方法研究[D].杨凌:西北农林科技大学, 2023.
WANG P.The development of nanobody and construction of immunoassay towards Vibrio parahaemolyticus[D].Yangling:Northwest A&F University, 2023.
[78] WANGMAN P, SURASILP T, PENGSUK C, et al.Development of a species-specific monoclonal antibody for rapid detection and identification of foodborne pathogen Vibrio vulnificus[J].Journal of Food Safety, 2021, 41(6):e12939.
[79] YANG G X, WEI C H, TANG Y Y, et al.A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination[J].Results in Chemistry, 2021, 3:100208.
[80] RODRIGUEZ-QUIJADA C, LYONS C, SANCHEZ-PURRA M, et al.Gold nanoparticle paper immunoassays for sensing the presence of Vibrio parahaemolyticus in oyster hemolymph[J].ACS Omega, 2023, 8(22):19494-19502.
[81] LIU Y S, ZHAO C, SONG X L, et al.Colorimetric immunoassay for rapid detection of Vibrio parahaemolyticus[J].Microchimica Acta, 2017, 184(12):4785-4792.
[82] ZHOU J, ZHANG C D, ZHANG X, et al.Immunomagnetic separation-based nanogold enhanced surface plasmon resonance and colloidal gold test strips for rapid detection of Vibrio parahaemolyticus[J].Archives of Microbiology, 2020, 202(5):1025-1033.
[83] WANG S, HU J H, XIAO S, et al.Fluorescent/electrochemical dual-signal response biosensing strategy mediated by DNAzyme-ferrocene-triggered click chemistry for simultaneous rapid screening and quantitative detection of Vibrio parahaemolyticus[J].Sensors and Actuators B:Chemical, 2023, 380:133393.
[84] ZHANG D Y, LIN H, CHEN L, et al.Cluster-bomb type magnetic biosensor for ultrasensitive detection of Vibrio parahaemolyticus based on low field nuclear magnetic resonance[J].Analytica Chimica Acta, 2023, 1248:340906.
[85] FARIDFAR G, ZEINODDINI M, AKBARZEDEHKOLAHI S, et al.Immunodiagnostic of Vibrio cholerae O1 using localized surface plasmon resonance (LSPR) biosensor[J].International Microbiology, 2021, 24(1):115-122.
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