Inflammation is a critical biological response to harmful stimuli, but chronic inflammation can lead to severe diseases such as cardiovascular disorders, diabetes, and cancer.This study explores the anti-inflammatory potential of bioactive peptides derived from porcine pancreatic hydrolysate, in a lipopolysaccharide (LPS)-induced systemic inflammation model in rats.Porcine pancreas, rich in proteins, was enzymatically hydrolyzed using papain to generate bioactive peptides.Rats were divided into protective and therapeutic intervention groups, receiving low (0.19 g/kg), medium (0.57 g/kg), and high (0.95 g/kg) doses of pancreatic hydrolysate before or after LPS challenge.Blood samples were collected after treatment, and inflammatory factors were assayed using ELISA kits.Histological analysis of colon tissues was performed using H&E.Results demonstrated that pancreatic hydrolysate significantly reduced pro-inflammatory cytokine levels in a dose-dependent manner, with the high-dose group showing the most pronounced effects.Protective intervention was more effective than therapeutic intervention in mitigating inflammation and preserving colonic morphology.This study highlights the potential of porcine pancreatic hydrolysate as a natural anti-inflammatory agent, offering a sustainable solution for valorizing meat industry by-products.
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