Food and Fermentation Industries >

2026 , Vol. 52 >Issue 14: 88 - 97

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044374

Efficient preparation and activity evaluation of Morchella esculenta glycoprotein base material

  • GU Yixiao ,
  • LI Wen ,
  • CHEN Wanchao ,
  • ZHANG Zhong ,
  • WU Di ,
  • LIU Peng ,
  • YANG Yan
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  • 1(College of Food Science, Shanghai Ocean University, Shanghai 201306, China)
    2(Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

Received date: 2025-09-02

  Revised date: 2025-11-26

  Online published: 2026-08-21

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Abstract

To systematically investigate the preparation process and potential biological activities of Morchella esculenta glycoprotein base material, a biological enzyme hydrolysis approach was adopted to prepare the M.esculenta glycoprotein base material.A comprehensive analysis and evaluation were performed, covering the contents of protein and polysaccharide components, amino acid and monosaccharide profiles, as well as functional activities including antihypertensive, antioxidant, and lipid-lowering effects.Single-factor experiments combined with response surface methodology (RSM) were utilized to determine the optimal preparation parameters for the glycoprotein-based material.The optimized conditions were as follows:enzymatic hydrolysis time of 56 min, pectinase dosage of 7%, solid-to-liquid ratio of 1∶30 (g∶mL), and hydrolysis temperature of 53 ℃.Under these optimized conditions, the resulting base material exhibited a protein content of (702.3±1.06) mg/g and a polysaccharide content of (153.5±0.09) mg/g.Amino acid analysis revealed the presence of 17 amino acids in the base material, with a total content of 140.19 mg/g, among which the total essential amino acids amounted to 37.89 mg/g.The monosaccharide composition of the base material is dominated by glucose, mannose, glucosamine, and galactose, with a total content of 208.55 mg/g.The half-maximal inhibitory concentration (IC50) values of the base material for DPPH free radical and ABTS cationic radical scavenging activities were 0.082 7 mg/mL and 0.261 1 mg/mL, respectively.Meanwhile, its IC50 value for angiotensin-converting enzyme (ACE) inhibition was 0.011 5 mg/mL, indicating promising antioxidant and antihypertensive activities.Additionally, the base material exhibited significant α-glucosidase inhibition, cholesterol esterase inhibition, and bile salt adsorption capacities, suggesting potential hypoglycemic and lipid-lowering effects.MS analysis identified two glycoprotein molecules in the base material, confirming that monosaccharides were linked to the serine and threonine residues of protein molecules via O-glycosylation.Molecular docking studies revealed that the glycoprotein molecules exhibited high-affinity binding to the ACE receptor, thereby exerting inhibitory activity.This study provides a theoretical foundation for the development and application of M.esculenta glycoprotein-based materials.

Key words： Morchella esculenta; glycoprotein; response surface methodology; preparation; antioxidant; lowering of blood pressure; molecular docking

Cite this article

GU Yixiao , LI Wen , CHEN Wanchao , ZHANG Zhong , WU Di , LIU Peng , YANG Yan . Efficient preparation and activity evaluation of Morchella esculenta glycoprotein base material[J]. Food and Fermentation Industries, 2026 , 52(14) : 88 -97 . DOI: 10.13995/j.cnki.11-1802/ts.044374

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