To explore the differences in the chemical composition of Citri Reticulatae Pericarpium at different harvest stages, to explain its efficacy material basis, and to provide a scientific basis for the differentiation and clinical application of Citri Reticulatae Pericarpium commodity specifications.Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were carried out on Citri Reticulatae Pericarpium at different harvest stages by LC-MS combined with multivariate statistical analysis.The Citri Reticulatae Pericarpium at different harvest stages could be divided into two categories, namely weihongpi and dahongpi, and 25 different components were screened out, including 18 flavonoids.The contents of obacunone, engeletin, eriocitrin, and naringin in the Dahongpi were significantly higher than those in the Weihongpi.The contents of quercetin-3,7,3′,4′-tetramethyl ether, gardenia b, 3,5,6,7,8,3′,4′-heptamethoxyflavone, isoliquiritin, scutellarein tetramethyl ether, tricin and ligustroflavone in weihongpi were significantly higher than those in dahongpi.The contents of poncirin, 7-[(3,3-dimethyloxiran-2-yl)methoxy]-8-[(3,3-dimethyloxiran-2-yl)methyl]-2H-1-benzopyran-2-one, obacunone, and cuparene changed the most, with a difference of 5 to 20 times between the highest and lowest values.This method can quickly and accurately analyze the chemical composition of Citri Reticulatae Pericarpium at different harvest stages, and explain the material basis of the biological activity differences between weihongpi and dahongpi from the perspective of chemical composition, which provides a scientific basis for the determination of the commodity specifications of Citri Reticulatae Pericarpium, a characteristic variety in Jiangxi Province, and provides a good reference for the determination of the harvest period of citrus plants.
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