Food and Fermentation Industries >

2026 , Vol. 52 >Issue 14: 326 - 334

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043116

Protective effect of guava fermentation broth against oxidative stress injury

  • LI Jinyong ,
  • LI Luyao ,
  • ZHANG Meng ,
  • SHI Doudou ,
  • ZHANG Jiachan ,
  • WANG Changtao
Expand
  • 1(School of Light Industry Science and Engineering, Beijing Technology and Business University,Beijing 138000,China)
    2(Beishang Jiamei (Beijing) Technology Co.Ltd., Beijing 100083, China)

Received date: 2025-04-27

  Revised date: 2025-11-10

  Online published: 2026-08-21

Fold

Abstract

In this study, guava fermentation fluid was produced by fermenting guava with lactic acid bacteria, and its antioxidant mechanism was investigated by determining the content of active substances before and after fermentation, combined with in vitro antioxidant experiments and cell models.The DPPH free radical and hydroxyl radical scavenging assays were used to assess antioxidant capacity.A model of oxidative damage was established by inducing H2O2 stress in human skin fibroblasts(HSFs), and cell survival, reactive oxygen species (ROS), catalase (CAT), malondialdehyde (MDA), collagen type I (COL-I), and hyaluronic acid (HA) levels were analyzed.Additionally, changes in the JAK-STAT signaling pathway were examined to explore its role in antioxidant defense.Results showed that the guava fermentation broth was rich in sugars and proteins.It exhibited strong DPPH and hydroxyl radical scavenging activity in vitro, compared with the model group, guava fermentation broth (10% volume fraction) significantly increased the levels of COL-I, CAT, and HA.It also decreased the accumulation of lipid peroxidation products (MDA and ROS) in HSFs damaged by H2O2-induced oxidative stress.This effect may be mediated through the regulation of the JAK-STAT signaling pathway, which counters oxidative stress damage caused by H2O2.

Key words： guava fermentation broth; H2O2 model; oxidative stress; pharmaceutical and cosmetic ingredients

Cite this article

LI Jinyong , LI Luyao , ZHANG Meng , SHI Doudou , ZHANG Jiachan , WANG Changtao . Protective effect of guava fermentation broth against oxidative stress injury[J]. Food and Fermentation Industries, 2026 , 52(14) : 326 -334 . DOI: 10.13995/j.cnki.11-1802/ts.043116

References

[1] 邝高波, 黄和.番石榴多酚体外抗氧化活性的研究[J].食品工业科技, 2014, 35(2):111-115.
KUANG G B, HUANG H.Study on antioxidant activity of guava polyphenols in vitro[J].Science and Technology of Food Industry, 2014, 35(2):111-115.
[2] 成宏斌, 李晓波, 贾笑英, 等.野菠萝果中多糖、黄酮与多酚的含量测定及抗氧化研究[J].现代食品, 2021, 27(1):83-89.
CHENG H B, LI X B, JIA X Y, et al.Determination of the content of polysaccharides, flavonoids and polyphenols in Pandanus tectorius Sol.and its antioxidant research[J].Modern Food, 2021, 27(1):83-89.
[3] 陈建福. 响应面优化超声辅助提取黄槿叶总黄酮工艺及其亚硝酸盐清除能力[J].食品工业科技, 2019, 40(6):193-197;204.
CHEN J F.Optimization of ultrasonic assisted extraction of total flavonoids from Hibiscus tilisceus leaves by response surface methodology and its scavenging ability of nitrite[J].Science and Technology of Food Industry, 2019, 40(6):193-197;204.
[4] 张山佳, 韩雪怡.沙葱总酚含量测定及抗氧化活性分析[J].中国调味品, 2022, 47(11):64-67.
ZHANG S J, HAN X Y.Determination of total phenol content and analysis of antioxidant activity of Allium mongolicum[J].China Condiment, 2022, 47(11):64-67.
[5] 池宁娟, 周虹, 高苏莉, 等.福林酚比色法测定转移因子胶囊中多肽的含量[J].中国药房, 2014, 25(17):1617-1618.
CHI N J, ZHOU H, GAO S L, et al.Content determination of the polypeptide in transfer factor capsules by folin-phenol colorimetry method[J].China Pharmacy, 2014, 25(17):1617-1618.
[6] 查学强, 王军辉, 潘利华, 等.石斛多糖体外抗氧化活性的研究[J].食品科学, 2007, 28(10):90-93.
ZHA X Q, WANG J H, PAN L H, et al.Study on antioxidant activity of polysaccharides from Dendrobium species[J].Food Science, 2007, 28(10):90-93.
[7] ADIGUN N S, OLADIJI A T, AJIBOYE T O.Antioxidant and anti-hyperlipidemic activity of hydroethanolic seed extract of Aframomum melegueta K.Schum in Triton X-100 induced hyperlipidemic rats[J].South African Journal of Botany, 2016, 105:324-332.
[8] 朱清华,邬家燕,杨丹,等.不同番石榴果实提取物抗氧化、降血糖活性比较[J].现代食品科技, 2024, 40(11):238-245.
ZHU Q H, WU J Y, YANG D, et al.Comparison of the antioxidant and hypoglycemic activities of different guava fruit extracts [J].Modern Food Science and Technology, 2024, 40(11):238-245.
[9] 杨斯惠, 马明芳, 曹亚楠, 等.不同杂粮多糖的体外抗氧化、益生和抗癌活性比较[J].食品工业科技, 2023, 44(1):1-10;47.
YANG S H, MA M F, CAO Y N, et al.Comparison of in vitro antioxidant, probiotic and anticancer activities of different coarse cereal polysaccharides[J].Science and Technology of Food Industry, 2023, 44(1):1-10;47.
[10] ALVAREZ-SUAREZ J M, GIAMPIERI F, GASPARRINI M, et al.Guava (Psidium guajava L.cv.red suprema) crude extract protect human dermal fibroblasts against cytotoxic damage mediated by oxidative stress[J].Plant Foods for Human Nutrition, 2018, 73(1):18-24.
[11] MAJOROS A, PLATANITIS E, KERNBAUER-HÖLZL E, et al.Canonical and non-canonical aspects of JAK-STAT signaling:Lessons from interferons for cytokine responses[J].Frontiers in Immunology, 2017, 8:29.
[12] FENG X Z, YANG Q Y, WANG C, et al.Punicalagin exerts protective effects against ankylosing spondylitis by regulating NF-κB-TH17/JAK2/STAT3 signaling and oxidative stress[J].BioMed Research International, 2020, 2020(1):4918239.
[13] TORO A, LAGE-VICKERS S, BIZZOTTO J, et al.Pin-pointing the key hubs in the IFN-γ pathway responding to SARS-CoV-2 infection[J].Viruses, 2022, 14(10):2180.
[14] ENO C O, ZHAO G P, OLBERDING K E, et al.The Bcl-2 proteins Noxa and Bcl-xL co-ordinately regulate oxidative stress-induced apoptosis[J].Biochemical Journal, 2012, 444(1):69-78.
[15] LI X, ZHANG N J, ZHANG Y, et al.E3 ligase Fbw7 participates in oxidative stress-induced myocardial cell injury via interacting with Mcl-1[J].Molecular Medicine Reports, 2019, 20(2):1561-1568.
[16] INOSHITA S, TAKEDA K, HATAI T, et al.Phosphorylation and inactivation of myeloid cell leukemia 1 by JNK in response to oxidative stress[J].Journal of Biological Chemistry, 2002, 277(46):43730-43734.
[17] HU L, CHEN L, YANG G Z, et al.HBx sensitizes cells to oxidative stress-induced apoptosis by accelerating the loss of mcl-1 protein via caspase-3 cascade[J].Molecular Cancer, 2011, 10(1):43.
[18] HIKITA H, KODAMA T, SHIMIZU S, et al.Bak deficiency inhibits liver carcinogenesis:A causal link between apoptosis and carcinogenesis[J].Journal of Hepatology, 2012, 57(1):92-100.
[19] HIKITA H, KODAMA T, TANAKA S, et al.Activation of the mitochondrial apoptotic pathway produces reactive oxygen species and oxidative damage in hepatocytes that contribute to liver tumorigenesis[J].Cancer Prevention Research, 2015, 8(8):693-701.
[20] RABIEI S, REZAEI M, NIKOO M, et al.Antioxidant properties of Klunzinger's mullet (Liza klunzingeri) protein hydrolysates prepared with enzymatic hydrolysis using a commercial protease and microbial hydrolysis with Bacillus licheniformis[J].Food Science and Technology International, 2022, 28(3):233-246.
[21] PRUDÊNCIO DE SOUZA E R, BRAZ M V D C, CASTRO R N, et al.Influence of microbial fermentation on the antioxidant activity of phenolic substances in Saccharomyces cerevisiae[J].Journal of Applied Microbiology, 2023, 134(8):lxad148.
[22] PU C H, SHI D D, INGRASSIA M, et al.Skincare benefits of a postbiotic ferment produced through djon djon mushroom fermentation by Saccharomyces[J].Journal of Cosmetic Dermatology, 2025, 24(2):e70067.
[23] CHENG W J, SHI X Q, ZHANG J C, et al.Role of PI3K-AKT pathway in ultraviolet ray and hydrogen peroxide-induced oxidative damage and its repair by grain ferments[J].Foods, 2023, 12(4):806.
[24] 张玉芝, 张永涛, 王海涛, 等.牡丹花发酵液功效活性检测及安全性评价[J].香料香精化妆品, 2022(5):5-10;23.
ZHANG Y Z, ZHANG Y T, WANG H T, et al.Efficacy detection and safety evaluation of peony flower fermentation liquid[J].Flavour Fragrance Cosmetics, 2022(5):5-10;23.
[25] 赵丹, 李萌, 苏宁, 等.枸杞发酵液的抗衰老活性和皮肤安全性研究[J].日用化学品科学, 2016, 39(6):24-27;37.
ZHAO D, LI M, SU N, et al.Evaluation of anti-aging activity and skin safety of fermented Lycium barbarum[J].Detergent & Cosmetics, 2016, 39(6):24-27;37.
[26] 张永涛, 赵丹, 安全, 等.青刺果发酵液对UVA损伤人皮肤成纤维细胞的修复作用[J].食品研究与开发, 2021, 42(23):26-32.
ZHANG Y T, ZHAO D, AN Q, et al.The repairing effect of the fermented liquid of Prinsepia utilis royle on human skin fibroblasts injured by UVA radiation[J].Food Research and Development, 2021, 42(23):26-32.
[27] LI S S, YANG M, CHEN Y P, et al.Dendritic cells with increased expression of suppressor of cytokine signaling 1(SOCS1) gene ameliorate lipopolysaccharide/d-galactosamine-induced acute liver failure[J].Molecular Immunology, 2018, 101:10-18.
[28] TIONG Y L, NG K Y, KOH R Y, et al.Melatonin prevents oxidative stress-induced mitochondrial dysfunction and apoptosis in high glucose-treated schwann cells via upregulation of Bcl2, NF-κB, mTOR, Wnt signalling pathways[J].Antioxidants, 2019, 8(7):198.
[29] LI P F, SHEN M H, GAO F, et al.An antagomir to microRNA-106b-5p ameliorates cerebral ischemia and reperfusion injury in rats via inhibiting apoptosis and oxidative stress[J].Molecular Neurobiology, 2017, 54(4):2901-2921.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.