Food and Fermentation Industries >

2026 , Vol. 52 >Issue 14: 351 - 357

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044828

Identification of geographic origin of Poria cocos polysaccharide by monosaccharide composition analysis

  • YANG Zixin ,
  • LIU Dongmei ,
  • AN Hongmei ,
  • WU Zhuying ,
  • AN Puchang ,
  • WANG Mengying ,
  • WU Haolin ,
  • LI Guohui ,
  • ZHONG Qiding
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  • 1(School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China)
    2(Technology Innovation Center of Light Industrial Consumer Goods Quality and Safety for State Administration for Market Regulation, Beijing 100015, China)
    3(Sinolight Technology Innovation Centre Co.Ltd., Beijing 100015, China)
    4(Sinolight Inspection and Certification (Taiyuan) Co.Ltd., Taiyuan 030400, China)
    5(College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China)

Received date: 2025-10-15

  Revised date: 2025-12-17

  Online published: 2026-08-21

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Abstract

This study established a reliable method for discriminating the geographical origin of Poria cocos based on the monosaccharide composition of its polysaccharides.Polysaccharides from samples collected from four major producing regions in China were hydrolyzed using microwave and analyzed by ion chromatography to determine the content of six monosaccharides.Chemometric techniques, including principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and linear discriminant analysis (LDA), were employed to evaluate the feasibility of origin traceability.The results indicated that the polysaccharides primarily consisted of fucose, glucosamine, glucose, galactose, xylose, and mannose, with distinct variations in their content and molar ratios among different origins.The OPLS-DA model clearly differentiated the samples from the four regions, demonstrating high goodness-of-fit and predictive ability (R2X=0.997, R2Y=0.909, Q2=0.879).Model stability was confirmed by a permutation test.Variable importance in projection (VIP) analysis identified glucose, fucose, and galactose as key discriminative markers.The LDA model achieved 100% correct classification rates in both initial and cross-validation groups.This study demonstrates that the monosaccharide composition fingerprint, combined with chemometrics, provides a reliable strategy for the geographical traceability and quality control of Poria cocos.

Key words： Poria cocos polysaccharides; monosaccharide composition; ion chromatography; microwave digestion; chemometrics

Cite this article

YANG Zixin , LIU Dongmei , AN Hongmei , WU Zhuying , AN Puchang , WANG Mengying , WU Haolin , LI Guohui , ZHONG Qiding . Identification of geographic origin of Poria cocos polysaccharide by monosaccharide composition analysis[J]. Food and Fermentation Industries, 2026 , 52(14) : 351 -357 . DOI: 10.13995/j.cnki.11-1802/ts.044828

References

[1] 张建逵, 窦德强, 王冰, 等.茯苓类药材的本草考证[J].时珍国医国药, 2014, 25(5):1181-1183.
ZHANG J K, DOU D Q, WANG B, et al.Textual research on the drugs of Poria[J].Lishizhen Medicine and Materia Medica Research, 2014, 25(5):1181-1183.
[2] LIU W W, YU L Z, CHEN Q, et al.Poria cocos polysaccharides alleviate obesity-related adipose tissue insulin resistance via gut microbiota-derived short-chain fatty acids activation of FGF21/PI3K/AKT signaling[J].Food Research International, 2025, 215:116671.
[3] PEI L N, FENG X, ZHANG L J, et al.Hypolipidemic activity and mechanisms of carboxymethyl pachymaran:Impact of the degree of substitution[J].Carbohydrate Polymers, 2025, 366:123890.
[4] YANG M Q, CUI B, HU S, et al.Poria cocos polysaccharides attenuate cardiac injury by inhibiting CaMKII-mediated P38/NF-κB/NLRP3 signaling pathway to reduce sepsis-induced apoptosis and inflammation[J].The American Journal of Chinese Medicine, 2025, 53(6):1865-1886.
[5] ZHU K, DU D S, SHI Y X, et al.Poria cocos polysaccharide delays aging by enhancing the antioxidant ability and suppressing the expression of the branched-chain amino acid transferase-encoding gene in Drosophila melanogaster[J].Journal of Agricultural and Food Chemistry, 2025, 73(15):9033-9046.
[6] 黄诗瑶, 孔亮, 王佳华, 等.茯苓多糖通过调控Akt/mTOR/SREBP-1c信号通路对非酒精性脂肪性肝病小鼠的改善作用[J].中成药, 2025, 47(1):58-65.
HUANG S Y, KONG L, WANG J H, et al.Effects of Poria cocos polysaccharides on improving mouse nonalcoholic fatty liver disease via regulation of Akt/mTOR/SREBP-1c signaling pathway[J].Chinese Traditional Patent Medicine, 2025, 47(1):58-65.
[7] LU J, TIAN J, ZHOU L, et al.Phytochemistry and biological activities of Poria[J].Journal of Chemistry, 2021, 2021:6659775.
[8] 刘珮瑶, 王琨, 梁杉, 等.茯苓多糖组成结构及生物活性研究进展[J].食品科学, 2023, 44(1):380-391.
LIU P Y, WANG K, LIANG S, et al.Advances in understanding the structure and biological activity of Poria cocos polysaccharides[J].Food Science, 2023, 44(1):380-391.
[9] 王珍, 董梁, 李家春, 等.茯苓产地适宜性分析研究[J].山东农业科学, 2011,43(12):14-18.
WANG Z, DONG L, LI J C, et al.Analysis on suitable producing areas of Poria cocos[J].Shandong Agricultural Sciences, 2011,43(12):14-18.
[10] 谢元彪, 李淞明, 詹常森.不同主产地栽培茯苓质量评价[J].中成药, 2023, 45(1):328-333.
XIE Y B, LI S M, ZHAN C S.Quality evaluation of Poria cocos cultivated in different main producing areas[J].Chinese Traditional Patent Medicine, 2023, 45(1):328-333.
[11] 邝嘉华, 黄燕燕, 胡金双, 等.解淀粉芽孢杆菌DMBA-K4高产胞外多糖的发酵条件优化及其抗氧化活性研究[J].食品与发酵工业, 2020, 46(22):28-35.
KUANG J H, HUANG Y Y, HU J S, et al.Optimization of fermentation conditions and anti-oxidation of exopolysaccharide produced by Bacillus amyloliquefaciens DMBA-K4[J].Food and Fermentation Industries, 2020, 46(22):28-35.
[12] 熊科胜, 檀华蓉, 葛宇, 等.高效液相色谱法测定油梨种子热处理后水溶性糖含量的变化[J].分析试验室, 2017, 36(7):794-798.
XIONG K S, TAN H R, GE Y, et al.Analysis of saccharides in avocado seed after heat treatment by high performance liquid chromatography[J].Chinese Journal of Analysis Laboratory, 2017, 36(7):794-798.
[13] 庄伟, 屈咪, 赵迪, 等.黑木耳多糖的结构组成及其免疫活性研究[J].食品科技, 2020, 45(2):205-210.
ZHUANG W, QU M, ZHAO D, et al.Structual composition and immunomodulatory activities of polysaccharides from Auricularia auricula[J].Food Science and Technology, 2020, 45(2):205-210.
[14] 何连军, 干雅平, 吕伟德, 等.高效阴离子交换色谱-脉冲安培检测法测定多花黄精多糖的单糖组成[J].中草药, 2017, 48(8):1671-1676.
HE L J, GAN Y P, LYU W D, et al.Monosaccharide composition analysis on polysaccharides in Polygonatum cyrtonema by high performance anion-exchange chromatography with pulsed amperometric detection[J].Chinese Traditional and Herbal Drugs, 2017, 48(8):1671-1676.
[15] 王梓轩, 李娅琦, 柳国霞, 等.高效阴离子交换色谱-脉冲安培检测法测定枸杞多糖的单糖及糖醛酸组成[J].中华中医药杂志, 2021, 36(10):6082-6085.
WANG Z X, LI Y Q, LIU G X, et al.Monosaccharide and uronic acid composition analysis on Lycium barbarum polysaccharides in Fructus Lycii by high performance anion-exchange chromatography with pulsed amperometric detection[J].China Journal of Traditional Chinese Medicine and Pharmacy, 2021, 36(10):6082-6085.
[16] 陈懿瑶, 罗阳, 闫燊, 等.HPAEC-PAD法测定石斛属植物单糖组分与主成分聚类分析[J].中国实验方剂学杂志, 2018, 24(15):106-111.
CHEN Y Y, LUO Y, YAN S, et al.Monosaccharide composition and principal component clustering analysis of Dendrobium by HPACE-PAD[J].Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(15):106-111.
[17] 张婷婷, 徐源, 杨淼硕, 等.板栗内壳多糖的提取工艺优化及其抗氧化功能和抗炎活性分析[J].饲料研究, 2025,48(16):93-98.
ZHANG T T, XU Y, YANG M S, et al.Optimization of extraction process for polysaccharides from Castanea mollissima endocarp and analysis of their antioxidant function and anti-inflammatory activities[J].Feed Research, 2025,48(16):93-98.
[18] SUKSOM W, WANNACHAI W, BOONCHIANGMA S, et al.Ion chromatographic analysis of monosaccharides and disaccharides in raw sugar[J].Chromatographia, 2015, 78(13-14):873-879.
[19] 任晓宇, 裴晓静, 张少云, 等.微波水解衍生高效液相色谱法测定饲料中的氨基酸[J].分析测试学报, 2017, 36(10):1255-1259.
REN X Y, PEI X J, ZHANG S Y, et al.Determination of amino acids in feed by high performance liquid chromatography with microwave hydrolysis derivatization[J].Journal of Instrumental Analysis, 2017, 36(10):1255-1259.
[20] 颉东妹, 王宁丽, 刘笑笑, 等.微波消解-离子色谱法测定枸杞多糖的含量及组成[J].食品安全质量检测学报, 2022, 13(4):1065-1072.
XIE D M, WANG N L, LIU X X, et al.Determination of the content and composition of Lycium barbarum polysaccharides by microwave digestion-ion chromatography[J].Journal of Food Safety & Quality, 2022, 13(4):1065-1072.
[21] LV Y Z, YANG Y J, CHEN Y, et al.Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Poria cocos[J].International Journal of Biological Macromolecules, 2024, 261:129878.
[22] 厉晓, 龚婕, 丁婵, 等.茯苓多糖的结构、活性及其构效关系研究进展[J].中草药, 2025, 56(1):305-317.
LI X, GONG J, DING C, et al.Research progress on structures, activities of Wolfiporia hoelen polysaccharides and its structure-activity relationship[J].Chinese Traditional and Herbal Drugs, 2025, 56(1):305-317.
[23] 黄小兰, 何旭峰, 杨勤, 等.PMP柱前衍生化HPLC法测定地参多糖的单糖组成[J].食品与发酵工业, 2020, 46(7):250-256.
HUANG X L, HE X F, YANG Q, et al.Determination of monosaccharide composition in Lycopus lucidus polysaccharides by pre-column PMP derivatization and HPLC[J].Food and Fermentation Industries, 2020, 46(7):250-256.
[24] 刘建香, 郭树芳, 雷宝坤, 等.绿肥生产利用方式对云南高原红壤理化性状及玉米产量的影响[J].植物营养与肥料学报, 2022, 28(2):237-246.
LIU J X, GUO S F, LEI B K, et al.Effects of green manure production and utilization on the physiochemical properties of red soil and maize yield in Yunnan Plateau[J].Journal of Plant Nutrition and Fertilizers, 2022, 28(2):237-246.
[25] 唐瑞, 赵明方, 李亚飞, 等.基于HPLC-DAD指纹图谱及含量测定结合化学计量法的不同产地麦冬质量评价[J].中华中医药杂志, 2025, 40(4):1930-1935.
TANG R, ZHAO M F, LI Y F, et al.Quality evaluation of Ophiopogonis Radix from different habitats based on HPLC-DAD fingerprint and content determination combined with stoichiometry[J].China Journal of Traditional Chinese Medicine and Pharmacy, 2025, 40(4):1930-1935.
[26] HU L F, WANG Y F, WU X, et al.Geographic origin discrimination and quantification of phenolic compounds and moisture in Artemisia argyi folium using NIRS and chemometrics[J].Journal of Agriculture and Food Research, 2025, 23:102295.
[27] 任娟, 刘晓, 李伟东, 等.基于指纹图谱和化学计量学的白芍不同炮制品成分差异研究[J].世界中医药, 2024, 19(14):2048-2054.
REN J, LIU X, LI W D, et al.Composition differences of different processed paeoniae Radix alba based on fingerprint and chemometrics[J].World Chinese Medicine, 2024, 19(14):2048-2054.
[28] 李平, 孙越鹏, 甄会贤, 等.基于HPLC多成分定量分析不同产地太子参药材的质量[J].中草药, 2023, 54(17):5734-5741.
LI P, SUN Y P, ZHEN H X, et al.Quality evaluation of Pseudostellariae Radix from different habitats by HPLC[J].Chinese Traditional and Herbal Drugs, 2023, 54(17):5734-5741.
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