Food and Fermentation Industries >

2026 , Vol. 52 >Issue 16: 307 - 314

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044852

Preparation technology of bacterial cellulose microspheres based on microfluidic in situ biosynthesis

  • QIAN Yuqing ,
  • CHEN Licheng ,
  • XIA Xinming ,
  • LIU Chunhuan ,
  • YANG Cheng
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  • 1(School of Chemical and Materials Engineering, Jiangnan university, Wuxi 214000, China)
    2(College of Food Science and Engineering, Ocean university of China, Qingdao 266404, China)

Received date: 2025-10-16

  Revised date: 2025-12-20

  Online published: 2026-09-10

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Abstract

Bacterial cellulose microspheres act as functional delivery carriers with high loading capacity, precise controlled-release performance and excellent biocompatibility.But the inability to achieve uniform preparation severely restricts their practical applications.Here, Gluconacetobacter xylinus was used as the cellulose-synthesizing strain to prepare bacterial cellulose microspheres by microfluidic water-in-oil (W/O) emulsion template method.The effects of emulsifier type, emulsifier concentration, and flow rate ratio on the formation and stability of microspheres were investigated.The results showed the prepared microspheres had an average size of 147 μm with the internal phase as 0.02 g/mL ultra-low gelling temperature agarose bacterial suspension, the receiving phase temperature at 4 ℃, the internal phase flow rate at 10 μL/min, and the external phase flow rate at 90 μL/min.G.xylinus grew inside the microspheres and secreted bacterial cellulose under these conditions.Overall, microfluidic technology enabled the successful in situ synthesis of bacterial cellulose microspheres by G.xylinus.This achievement provides a reliable strategy for the large-scale production of bacterial cellulose microspheres and further expands their application scope in the food industry.

Key words： microfluidics; in situ biosynthesis; emulsion template method; bacterial cellulose microspheres; preparation process

Cite this article

QIAN Yuqing , CHEN Licheng , XIA Xinming , LIU Chunhuan , YANG Cheng . Preparation technology of bacterial cellulose microspheres based on microfluidic in situ biosynthesis[J]. Food and Fermentation Industries, 2026 , 52(16) : 307 -314 . DOI: 10.13995/j.cnki.11-1802/ts.044852

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