Exopolysaccharides (EPS) are high-molecular-weight carbohydrate polymers secreted by microorganisms and possess various biological activities.Although Leuconostoc mesenteroides is a well-known EPS-producing lactic acid bacterium, the structure-function relationship between its EPS structures and their regulatory effects on gut microbiota remains poorly understood.This study characterized the basic structures of EPS derived from 13 strains of L.mesenteroides and identified the most pronounced structural differences between the EPS produced by DQHXN_Q37M5 and DQHXN_Q38M5.To investigate the structure-activity relationship between these EPS variants and gut microbiota modulation, an antibiotic-associated diarrhea model was established.The results demonstrated that DQHXN_Q38M5 EPS was more effective in alleviating diarrhea symptoms and restoring intestinal barrier integrity.Although both polysaccharides exerted targeted modulatory effects on gut microbiota in vivo, the specific bacterial genera enriched by DQHXN_Q38M5 EPS showed stronger synergy with the improvement of host physiological indicators.To further validate the direct enrichment of specific bacterial genera, this study conducted in vitro anaerobic fermentation using each EPS as the sole carbon source.The results confirmed that the two EPS types targeted distinct microbial groups and exhibited significant dose-dependent effects.Specifically, Alistipes and Enterococcus were preferentially stimulated by DQHXN_Q37M5 EPS, whereas Parabacteroides and Parasutterella responded more strongly to DQHXN_Q38M5 EPS consistent with the in vivo trends.These genera are suggested as potential key mediators in EPS-driven microbial restructuring and functional responses.This study enhances the understanding of EPS structure-function relationships and provides a scientific basis for the targeted application of L.mesenteroides in the probiotic industry.
ZHAO Yingqi
,
QIAN Xin
,
WANG Jingyu
,
JIN Zhiying
,
SUN Jingge
,
TIAN Peijun
,
ZHANG Hao
. Inter-strain variability in exopolysaccharides produced by Leuconostoc mesenteroides and their modulatory effects on gut microbiota[J]. Food and Fermentation Industries, 2026
, 52(16)
: 271
-279
.
DOI: 10.13995/j.cnki.11-1802/ts.044818
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