Regulation of microbial community structure and metabolism in rice-flavor Baijiu fermentation by yeast-fortified Xiaoqu

  • LEI Yu ,
  • YUE Dehong ,
  • LIAO Zuyue ,
  • QIN Zeguang ,
  • ZHENG Yifu ,
  • WU Qun
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  • 1(Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(Guilin Xiangshan Distillery Co.Ltd., Guilin 541500, China)

Received date: 2025-11-14

  Revised date: 2026-01-06

  Online published: 2026-09-21

Abstract

The quality of rice-flavor Baijiu is highly dependent on Xiaoqu fermentation.However, the microbial community in the traditional natural fermentation system is complex and greatly influenced by the environment, especially the stability of yeast community is easily affected.Among them, yeast can significantly affect the community dynamics and stability of the fermentation system by mediating resource competition and metabolic interaction, which leads to the difficulty in controlling flavor metabolism in the fermentation process and restricts the improvement of Baijiu quality.In order to solve the above problems, this study fortified the Xiaoqu by incorporating Pichia kudriavzevii and Wickerhamomyces anomalus during the traditional production process, and applied the fortified Xiaoqu in Baijiu fermentation.The results showed that the yeast diversity of fortified Xiaoqu was significantly increased, and the microbial community structure of the fermentation system could be effectively optimized after the application of fortified Xiaoqu.The network analysis showed that the microbial interaction increased during the fermentation process.Flavor detection and metabolic function prediction show that yeast-fortified Xiaoqu can significantly increase the content of esters in the fermentation system by enhancing the synthesis of carbonylesterase in yeast community.This study provides theoretical basis and technical support for the modernization of traditional fermentation technology of rice-flavor Baijiu.

Cite this article

LEI Yu , YUE Dehong , LIAO Zuyue , QIN Zeguang , ZHENG Yifu , WU Qun . Regulation of microbial community structure and metabolism in rice-flavor Baijiu fermentation by yeast-fortified Xiaoqu[J]. Food and Fermentation Industries, 2026 , 52(17) : 102 -111 . DOI: 10.13995/j.cnki.11-1802/ts.045175

References

[1] 余洁瑜, 刘功良, 白卫东, 等. 米香型白酒的研究进展[J]. 中国酿造, 2021, 40(1):29-32.
Yu J Y, Liu G L, Bai W D, et al. Research progress on rice-flavor Baijiu[J]. China Brewing, 2021, 40(1):29-32.
[2] Hao F, Tan Y W, Lyu X B, et al. Microbial community succession and its environment driving factors during initial fermentation of Maotai-flavor Baijiu[J]. Frontiers in Microbiology, 2021, 12:669201.
[3] Jin Y, Li D Y, Ai M, et al. Correlation between volatile profiles and microbial communities: A metabonomic approach to study Jiang-flavor liquor Daqu[J]. Food Research International, 2019, 121:422-432.
[4] Ye H, Wang J, Shi J, et al. Automatic and intelligent technologies of solid-state fermentation process of Baijiu production: Applications, challenges, and prospects[J]. Foods, 2021, 10(3):680.
[5] Yan Z, Zheng X W, Chen J Y, et al. Effect of different Bacillus strains on the profile of organic acids in a liquid culture of Daqu[J]. Journal of the Institute of Brewing, 2013, 119(1-2):78-83.
[6] Guo J H, Sun L H, Guo H W, et al. Effect of cellulase-producing bacteria on fungi community structure and ester generation in Chinese liquor fermenting grains[J]. Journal of the American Society of Brewing Chemists, 2018, 76(2):130-140.
[7] Zhang P P, Liu Y B, Li H D, et al. Strategies and challenges of microbiota regulation in Baijiu brewing[J]. Foods, 2024, 13(12):1954.
[8] Tang J, Liu Y C, Lin B, et al. Effects of ultra-long fermentation time on the microbial community and flavor components of light-flavor Xiaoqu Baijiu based on fermentation tanks[J]. World Journal of Microbiology and Biotechnology, 2021, 38(1):3.
[9] 贾丽艳, 张丽, 李惠源, 等. 果香风味导向的库德毕赤酵母FJZ的分离鉴定及生物学特性研究[J]. 中国食品学报, 2021, 21(1):276-282.
Jia L Y, Zhang L, Li H Y, et al. The screening and identification of Pichia kudriavzevi FJZ by flavor-oriented technology and it’s biological characteristics[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21(1):276-282.
[10] 王德培, 胡阳, 焦富. 库德里阿兹威氏毕赤酵母在发酵工业中的研究进展[J]. 酿酒科技, 2021(6):95-101.
Wang D P, Hu Y, Jiao F. Research progress in the application of Pichia kudriavzevii in industrial fermentation[J]. Liquor-Making Science & Technology, 2021(6):95-101.
[11] Alonso-Del-Real J, Lairón-Peris M, Barrio E, et al. Effect of temperature on the prevalence of Saccharomyces non cerevisiae species against a S. cerevisiae wine strain in wine fermentation: Competition, physiological fitness, and influence in final wine composition[J]. Frontiers in Microbiology, 2017, 8:150.
[12] 吕想, 高甜甜, 刘伟, 等. 基于GC-IMS分析非酿酒酵母与酿酒酵母混合发酵脐橙酒挥发性香气成分[J]. 食品工业科技, 2023, 44(17):139-148.
Lyu X, Gao T T, Liu W, et al. Analysis of volatile aroma components of navel orange wine produced by mixed fermentation of non Saccharomyces cerevisiae and Saccharomyces cerevisiae based on GC-IMS[J]. Science and Technology of Food Industry, 2023, 44(17):139-148.
[13] 卜光明, 周化斌, 周茂洪, 等. 酿造酒中非酿酒酵母的研究进展[J]. 食品工业科技, 2019, 40(14):346-352.
Bu G M, Zhou H B, Zhou M H, et al. Research progress on the non-Saccharomyces in the brewing wine fermentation[J]. Science and Technology of Food Industry, 2019, 40(14):346-352.
[14] 赵志军, 王瑞卿, 刘延波, 等. 酿酒糟醅中生香酵母的筛选及培养条件[J]. 食品与生物技术学报, 2020, 39(9):61-67.
Zhao Z J, Wang R Q, Liu Y B, et al. Screening of aroma-producing yeast in distiller’s grains and optimization of culture conditions[J]. Journal of Food Science and Biotechnology, 2020, 39(9):61-67.
[15] 王云, 班世博, 吴群, 等. 米香型白酒酿造过程微生物群落及其代谢功能特征[J]. 食品与发酵工业, 2024, 50(24):151-158.
Wang Y, Ban S B, Wu Q, et al. Characteristics of microbial communities and metabolic functions during brewing of rice-flavor Baijiu[J]. Food and Fermentation Industries, 2024, 50(24):151-158.
[16] 曹玉发, 孙怡辰, 魏轩, 等. 3个年份浓香型白酒香气成分比较研究[J]. 食品安全质量检测学报, 2021, 12(2):769-779.
Cao Y F, Sun Y C, Wei X, et al. Investigation on the aroma compounds of strong-aroma-type Baijiu in 3 different aging years[J]. Journal of Food Safety & Quality, 2021, 12(2):769-779.
[17] 胡晓龙, 余苗, 王康丽, 等. 浓香型白酒窖泥微生物群落多样性及理化因素对其影响[J]. 食品研究与开发, 2021, 42(2):178-185; 204.
Hu X L, Yu M, Wang K L, et al. Diversity of microbial community and its correlation with physicochemical factors in Luzhou-flavor liquor pit mud[J]. Food Research and Development, 2021, 42(2):178-185; 204.
[18] 沈怡方. 白酒生产技术全书[M]. 第三版. 北京: 中国轻工业出版社, 2005.
Shen Y F. Encyclopedia of Baijiu production technology[M]. 3rd ed. Beijing: China Light Industry Press, 2005.
[19] Wang Z, Ji X A, Wang S L, et al. Sugar profile regulates the microbial metabolic diversity in Chinese Baijiu fermentation[J]. International Journal of Food Microbiology, 2021, 359:109426.
[20] Wang P, Wu Q, Jiang X J, et al. Bacillus licheniformis affects the microbial community and metabolic profile in the spontaneous fermentation of Daqu starter for Chinese liquor making[J]. International Journal of Food Microbiology, 2017, 250:59-67.
[21] Mo X L, Fan W L, Xu Y. Changes in volatile compounds of Chinese rice wine wheat Qu during fermentation and storage[J]. Journal of the Institute of Brewing, 2009, 115(4):300-307.
[22] Levin R A, Myers N R, Bohs L. Phylogenetic relationships among the “spiny solanums” (Solanum Subgenus Leptostemonum, Solanaceae)[J]. American Journal of Botany, 2006, 93(1):157-169.
[23] Zhang X L, Zhang R N, Jiang Z W, et al. Biodiversity of the bacteria closely associated with the toxigenic dinoflagellate, Alexandrium minutum, and screening of its toxigenic bacteria strains[J]. Toxicon, 2019, 158: S6.
[24] Wang S L, Wu Q, Nie Y, et al. Construction of synthetic microbiota for reproducible flavor compound metabolism in Chinese light-aroma-type liquor produced by solid-state fermentation[J]. Applied and Environmental Microbiology, 2019, 85(10): e03090-e03018.
[25] Edgar R C, Haas B J, Clemente J C, et al. UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics, 2011, 27(16):2194-2200.
[26] Rognes T, Flouri T, Nichols B, et al. VSEARCH: A versatile open source tool for metagenomics[J]. PeerJ, 2016, 4: e2584.
[27] Deng N, Du H, Xu Y. Cooperative response of Pichia kudriavzevii and Saccharomyces cerevisiae to lactic acid stress in Baijiu fermentation[J]. Journal of Agricultural and Food Chemistry, 2020, 68(17):4903-4911.
[28] 石馨, 惠明, 田青, 等. 异常威客汉姆酵母产酯酶培养基优化及发酵液香气物质分析[J]. 发酵科技通讯, 2021, 50(3):149-155.
Shi X, Hui M, Tian Q, et al. Optimization of esterase production medium and analysis of aromatic substances in the fermented broth by Wickerhamomyces anomalus[J]. Bulletin of Fermentation Science and Technology, 2021, 50(3):149-155.
[29] 明红梅, 周健, 陈蒙恩, 等. 大曲中异常威克汉姆酵母发酵产苯乙醇的条件优化[J]. 湖北农业科学, 2015, 54(14):3492-3496.
Ming H M, Zhou J, Chen M E, et al. Optimization of phenylethanol fermentation conditions of Wickerhamomyces anomalus from Daqu by response surface methodology[J]. Hubei Agricultural Sciences, 2015, 54(14):3492-3496.
[30] 刘晓柱, 黎华, 李银凤, 等. 接种异常威克汉姆酵母对空心李果酒理化特性及香气组分的影响[J]. 食品科技, 2020, 45(11):21-27.
Liu X Z, Li H, Li Y F, et al. Effects of inoculation Wickerhamomyces anomalus on the physicochemical property and aroma profile of Prunus salicina Lindl. Cv ‘kongxinli’ fruit wine[J]. Food Science and Technology, 2020, 45(11):21-27.
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