Diversity and formation mechanism of the microflora in strong flavor Baijiu Baobaoqu
ZHANG Qian1, HAN Baolin1,2, LI Zijian1, XIE Jun1,2, YU Dong3, ZOU Yongfang3, GUO Huixiang3, WEN Jing3, ZHANG Lingling1, LUO Huibo1,2*, HUANG Dan1,2*
1(College of Bioengineering,Sichuan University of Science & Engineering,Zigong 643000,China) 2(Liquor Making Biotechnology and Application Key Laboratory of Sichuan Province,Yibin 644000,China) 3(Shede Spirits Co.Ltd.,Suining 629209,China)
Abstract: The structural succession rule of the microflora of Qupi (representing the surface of Baobaoqu, marked as QP below) and Quxin (representing the interior of Baobaoqu, marked as QX below) in the process of the spontaneous solid-state fermentation (SSF) of Baobaoqu was studied. The correlation between the microflora structure and the physicochemical factors of QP and QX, the diversity of microflora and the formation mechanism of diversity endowed by the unique structure of Baobaoqu were also analyzed. The pH, total acid, temperature, and moisture of QP and QX in the process of SSF were measured, the microflora structure in QP and QX was studied by high-throughput sequencing method, and the difference of microflora between QP and QX was analyzed by linear discriminant analysis effect size (LEfSe). Redundancy analysis, mantel test analysis, and Pearson correlation analysis were used to analyze the correlation between the physicochemical factors and the microflora of Baobaoqu. The results showed that there were significant differences in the bacterial and fungal communities between these two groups. Lactobacillus, Weissella, Rhizopus, Rhizomucor, Pichia, Aspergillus had higher abundances in QP. The abundance of Thermoascus, Pseudomonadales, Rhizobiales, Saccharomycetales, and Sporidiobolaleswas higher in QX. Meanwhile, it was also found that pH, total acid, temperature, and moisture were important factors affecting the microbial composition of QP and QX. The results revealed the characteristics and differences of microflora structure in QP and QX during the Baobaoqu SSF process, which might be helpful to the analysis of the formation mechanism of microbial diversity in Baobaoqu.
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2021, Vol. 47 
