Food and Fermentation Industries >

2010 , Vol. 36 >Issue 01: 142 - 145

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.01.020

Food and Fermentation Industries

Identification of Fish Derived Material in Food

  • Zhang Shuya ,
  • Jin Liqin ,
  • Jiang Jianxiong ,
  • Li Ni ,
  • Zhang Jiayi ,
  • Zhang Xiaofeng
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Online published: 2010-01-25

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Abstract

A specific and sensitive PCR method of fish derived material in food was developed.Primers fish1 and fish2 amplified a 224bp fragment from 35 fish samples,whereas no amplification was obtained from 24 other animal samples and 10 plant samples.The detection limit of the assay was 0.1ng DNA and 0.1%(W/W).80 food samples were analyzed by PCR,fish derived material were labeled incorrectly in 13 samples.The detection method can be used to identify fish derived material in food.

Key words： feedstuff; fish derived material; PCR

Cite this article

Zhang Shuya , Jin Liqin , Jiang Jianxiong , Li Ni , Zhang Jiayi , Zhang Xiaofeng . Identification of Fish Derived Material in Food[J]. Food and Fermentation Industries, 2010 , 36(01) : 142 -145 . DOI: 10.13995/j.cnki.11-1802/ts.2010.01.020

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