Barley is the most important raw material for beer production.Its proteins are essential for the quality of the malt and beer.In this paper,sodium dodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Bradford methods were used to investigate the heat-stable water-soluble proteins from germinated barley under salt stress and to monitor the change in secreted protease.The result showed that protease activity was inhabited under sodium salt stress,and the heat-stable water-soluble protein content was not increased obviously.In contrast,the protease activity was not restrained and the heat-stable water-soluble protein content increased remarkably under low concentration calcium salt stress.However,their activities were weakened and the heat-stable water-soluble protein content was not increased significantly under high concentration calcium salt stress.In summary,the protease could be secreted normally but their activities were affected under salt stress,and that protein anabolism of germinated barley was inhibited under high concentration salt stress.