The pure fermentation experiments which simulated the fermentation conditions were carried out among the microbes isolating from the whole brewing process.The results indicated that the main metabolites included acetaldehyde,ethanol,ethyl acetatet,lactic acid and acetic acid by GC and HPLC.30 functional microbes were identified by metabolic control fermentation experiments on substance level,which belonged to Pichia,Osporium,Saccharomyces,Bacillus,Lactobacillus,Corynebacterium etc,respectively.The results could provide important theoretical evidence and microbes resource to the exploration of the brewing mechanism and optimize the brewing technique.