Using biomass growth multiple and cell density as major indices,Lac101 and Leuconostoc oneos 6066 as compared strains,the tolerances to SO2,ethanol,acid,temperature and MLF activities of twelve strains isolated from loquat wine were studied.The results indicated that Lactobacillus R23,Lactococcus R33 and R35 showed higher comprehensive abilities,which superior to Lac101 and 6066,three strains could keep alive at 120 mg/L SO2,13% alcohol degree,pH value 3.0,the biomass reached 109 CFU/mL at 18℃,and their malolactic fermentaion activities were above 100u.Accordingly,R23,R33 and R35 were the excellent lactic acid bacteria for the MLF of loquat wine.