Food and Fermentation Industries >

2010 , Vol. 36 >Issue 03: 184 - 186

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.03.041

Food and Fermentation Industries

Extraction of Soluble Protein from Jerusalem artichoke and Determination of Its Molecular Weight

  • Li Xueyan ,
  • Zhang Wei ,
  • Zhang Xiulan ,
  • Li Zhizhong
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Online published: 2010-03-25

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Abstract

The soluble protein of Jerusalem artichoke was extracted by the phosphate buffer. Single experiment and orthogonal experiment were used to discuss the optimized extraction,and the SDS-PAGE was used to analyze furthermore. The results showed that the optimal extraction conditions are as follows:ratio of material to solution 1:40(w/v),extraction time 4h,temperature 35℃,pH8.0. Under this condition,the average extracting rate of soluble protein was 14.7mg/g. In the SDS-PAGE,the molecular weights of these proteins were between 20.9 ku and 69.2 ku.

Key words： Jerusalem artichoke; protein; extraction; SDS-PAGE; molecule weight

Cite this article

Li Xueyan , Zhang Wei , Zhang Xiulan , Li Zhizhong . Extraction of Soluble Protein from Jerusalem artichoke and Determination of Its Molecular Weight[J]. Food and Fermentation Industries, 2010 , 36(03) : 184 -186 . DOI: 10.13995/j.cnki.11-1802/ts.2010.03.041

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