A rapid method was developed for determination and confirmation of virginiamycin M1 residue in chicken muscle,chicken liver,chicken kidney,swine kidney,and bovine kidney using liquid chromatography/tandem mass spectrometry (LC-MS/MS).The virginiamycin M1 residues in samples were extracted with acetonitrile,then diluted with water and cleaned up with hexane.The sample was analyzed by LC-MS/MS in positive mode,with a YMC-Pack Pro C18 column as the analytical column.The limit quantitation (LOQ) was 25 μg/kg for chicken muscle,100 μg/kg for chicken liver,100 μg/kg for chicken kidney,150 μg/kg for swine kidney,and 100 μg/kg for bovine kidney respectively.The recoveries were 86.8%-108 % for chicken muscle,81.8%-109% for chicken liver,85.5%-102% for chicken kidney,82.7%-102% for swine kidney,and 80.7%-103% for bovine with the relative standard deviations of 4.3%-6.7 % for chicken muscle,3.3%-7.3% for chicken liver,2.8%-4.6% for chicken kidney,1.9%-4.1% for swine kidney,and 3.4%-6.9% for bovine kidney.The method is simple,rapid,and accurate,and its performance meets the requirements of the domestic and international food safety regulations.The method is applicable for determination and confirmation of virginiamycin M1 residue in animal tissues.
Chen Xiaoxia
,
Yue Zhenfeng
,
Ye Weixiang
,
Zhao Fengjuan
,
Rao Li
,
Ge Liya
,
Ouyang Shan
. Rapid Determination of Virginiamycin M1 Residue in Animal Tissue Using Liquid Chromatography/Tandem Mass Spectrometry[J]. Food and Fermentation Industries, 2010
, 36(04)
: 175
-178
.
DOI: 10.13995/j.cnki.11-1802/ts.2010.04.012