To optimize complex enzyme combined with ultrasonic wave extraction of pumpkin polysaccharides,the response surface methodology(RSM)was adopted.The experiment was based on two separate extraction methods and synergistic effect on the extraction was studied compared with the two independent methods.Raw materials were carried by ultrasonic treatment after complex enzyme hydrolysis.Based on the single factor experiment,the mathematical regression model was established about the dependent variable(extraction yield of pumpkin polysaccharides)and independent variables(working time,ultrasonic power and ratio of water to material).The factors influencing the process parameters were determined by regression analysis,and the results showed that the optimum conditions for the ultrasonic extraction were as follows:working time=12 min,ultrasonic power = 300W,ratio of water to material=22∶1(mL/g)and the extraction yield of pumpkin polysaccharides=22.80% under this condition.The actual value was 22.77%.