Enzymatic hydrolyzing technology of corn gluten meal was investigated.The results showed that the optimum conditions are: pH 8.0,temperature 65℃,enzyme concentration 4 000 U/g,substrate concentration 2%,and hydrolyzing time 2 h.Under the optimizing technology parameters,the degree of hydrolysis was 73.25%.The hydrolysate were separated by Amberlite CG-120 and Sephadex G-25.Most of DPPH savenging activity profiles were deserved and separated by RP-HPLC twice,and a 1147.2 Da peptide was deserved with higher purity.