This study evaluated the sterilizing effects of pulsed electric fields(PEF) on E.coli in carrot juice sample and its impact on the carotenoid content.The results showed that pulsed electric intensity,treatment time and temperature were the most influencing factors of PEF treatment.With PEF treatment at 30 kV/cm,500 μs at 4 ℃,the reduction of E.coli is 5.9 lg.The sterilizing effect significantly increased with stronger pulsed electric intensity and longer treatment time.When raising the temperature to 20 ℃ the reduction increased by 1.0 log.PEF treatments can significantly increase the carotenoid content of carrot juice.With PEF treatment at 30 kV/cm,500 μs,carotenoid content increased by 55.66% compared to the sample before treatment.