Denaturation of protein induced by lipoxygenase catalyzed linoleic acid in the model system was investigated by UV,fluorescence and Fourier transform infrared(FT-IR)spectrometer,etc.The results indicated that aromatic amino acid residue and the surface hydrophobicity increased,sulfhydryls(SHs)content decreased,and secondary structure changed obviously.This showed the change in protein structure was triggered by inducing of lipid oxidation.