The antioxidant crude extracts of Defatted rice bran were separated into three subfractions(ethyl acetate fraction,n-butanol fraction and aqueous fraction)by organic solvents of different polarities based on liquids-liquids extracting method.Their antioxidant activities were evaluated by Fe3+to Fe2+-reducing activity,1,1-diphenyl-2-picrylhydrazyl(DPPH)and superoxide anion free radical-scavenging assay,metal chelate ability.The results indicate that Defatted rice bran possesses powerful antioxidant ability.The n-butanol fraction have the strongest antioxidant activity;with high levels of total flavonoids content,total phenol contents,reductive activity,respectively.These results suggest that Defatted rice bran antioxidant enriched in n-butanol extract.