Garlic oil was extracted by Endogenous Enzyme and steam-distillation method.We also studied the effect of the relative parameters on garlic oil extraction and optimization of the process conditions.The relevant parameters were time and temperature of enzymolysis and distillation,respectively.Based on optimization condition,we analyzed antioxidant activity of garlic oil.The results were as follows:Enzymolysis time and temperature was 100 min and 30℃,and time and temperature of distillation was 80min and 90℃.The antioxidant ability of garlic oil was identical to that of VE.