Native wine yeasts isolated from spontaneously fermenting Beihong red wine grape(Muscat Hamburg×V.amurensis) were plated onto Wallerstein Laboratory Nutrient Agar(WL).Seven unique colony morphologies were identified and one strain showed distinct character of Saccharomyces cerevisia.Further identification by micro-morphological observation,physiological and biochemical experiment and DNA sequence analysis also confirmed the strain as S.cerevisia.The model synthetic medium which could simulate a standard grape juice was used to study the important enological traits of the newly selected S.cerevisia,the results showed that the newly selected S.cerevisia had high ethanol production,high fermentation activity and high glycerol production.The model synthetic medium was modified to study the stress tolerance of the newly selected S.cerevisia,the result showed that the modified model synthetic medium could be used for analyzing single stress tolerance of wine yeast,the newly selected wine yeast could tolerant high temperature,high ethanol concentration,high sorbitol concentration and low pH.Pilot scale fermentation using nature grape juice also showed that the newly selected S.cerevisia had desirable fermentative behavior.