In order to better understand the correlation between bacterial community of high-temperature DaQu and air of Qu workshop of multi-grain strong-flavor liquor factories,and to collect potential microbial resources,we employed modified nutrient agar medium and Gogan-I medium for isolation and cultivation.Then 16S rRNA gene sequences of 217 non-redundant bacterial isolates were be analyzed,and abilities of lignocellulose-degrading,saccharifying,7 % ethanol and pH 4.5 tolerance of these isolates were be screened,too.Phylogenetic analysis based on 16S rRNA gene sequences showed that Bacillus is the most dominant genera of two bacterial community,and Staphylococcus and Streptomyces is following dominant genera of high-temperature DaQu and air of Qu workshop,respectively.These strains with abilities of lignocellulose-degrading,saccharifying,ethanol tolerance and structure of two bacterial communities were comparatively analyzed.The results indicated that Qu workshop bacterial community and climatic factors remarkably influenced the structure and function of high-temperature DaQu bacterial community.