The optimized cultural condition and cultural medium of fermentation to produce the high SAM producing baker's yeast was studied.The results of single factor experiment showed that the most favorable pH value,medium volumes,inoculums size is 5.0,70/250 mL,2%.The results of orthogonal design experiments showed that the most favorable cultural medium ingredients are 12°Brix molasses,6.667g/L yeast powder,2.829g/L(NH4)2SO4,0.2g/L MgSO4,0.1g/L ZnSO4 and 1g/L KH2PO4.Under the optimal condition,SAM yield is 14.26 mg/L and content is 1.64 mg/g.It increased 76.5% and 84.3%,respectively.