Wheat Qu plays an important role in the fermentation of Yellow rice wine.It provides various enzymes(e.g.hydrolase) and flavor compounds precursor to Chinese rice wine during fermentation.In this paper,according to the theory of metaproteomics,three different preparation methods of sample,TCA-acetone precipitation,acetone precipitation and 2-D Cleanup Kit were used to isolate the metaproteome from Yellow rice wine wheat Qu extract,respectively.The results were compared by using two-dimensional(2-D) electrophoresis.It demonstrated that the method of using 2-D Cleanup Kit was superior to the other two methods in the aspects of solubility,purity and separating resolution with the metaproteome samples.In conclusion,a suitable preparation method for metaproteome isolation from Yellow rice wine wheat Qu extract was established and the 2-DE maps with good quality were obtained.It laid a certain foundation for further characterization of the metaproteome by mass spectrometry analysis with enhanced accuracy.