Aspergillus oryzae AS3.951 was inoculated into Ostreagigas thunberg meat homogenate in order to excrete protease and amylase to hydrolyze oyster meat.The effects of fermentation conditions on the yield of soluble protein and glycogen were studied using a one-at-a-time strategy and orthogonal experimental design.The optimal conditions were: protein concentration 15%,inoculums 20%,initial pH 7.0,140 r/min,30 ℃ for 48 h.In the optimal condition,the yield of soluble protein and glycogen reached 85.33% and 90.52% respectively.The results show that preparing oyster hydrolysates using Aspergillus oryzae fermentation was practical and may lay the foundation for the study of fermentation conditions to improve the yield of soluble protein and glycogen with statistic methods.