In order to improve the lipid production of cassava starch,the process of cassava starch hydrolysis(CSH) was optimized using orthogonal experimental design and Uniform design.The results showed that the content of α-amylase and glucoamylase and the liquefaction temperature significantly influenced cassava starch amylolysis.The dextrose equivalent value was up to 97.3% when content of α-amylase and glucose amylase and liquefaction temperature were 756 u/g,602 u/g and 92℃,respectively.While the CSH was used as a carbon source,the biomass and total lipid content of Trichosporon cutaneum were 16.38g/L and 7.22 g/L respectively,which increased by 46.25% and 41.12% as compared with those using glucose as a carbon source.Therefore,cassava starch hydrolysis,as a novel carbon source,is an ideal route to produce microbial lipid.