Four factors including ethanol concentration,solvent to material ratio,extraction temperature and extraction time were studies on the yield of hesperidin from the citrus peel by using single factor analysis method.Then a four factor,three-level Box-Behnken central composite design(CCD) coupled with response surface analysis(RSM) was applied in optimization of the extraction.It was found that the optimum extraction conditions were: ethanol concentration 71%,solvent to material ratio 22∶ 1(mL/g),extraction temperature 70℃ and extraction time 2.5 h.Under the optimized extraction conditions,hesperidin extraction yield was 4.82%.The research on anti-oxidation properties showed that the extracted hesperidin had scavenging activity on free hydroxyl radical.