Resveratrol and anthraquinone were extracted and separated from Polygonum cuspidatum Sieb.et.Zucc.Methanol hot reflux orthogonal test was applied.Mixed solvent was used in purification and 50% ethanol was used in separation.The optimum conditions of extraction were: 98% methanol,solid-liquid ratio 1∶ 6,extracted 3 times and 1h/ each time.As a result,the content of resveratrol was 1.912% and the content of anthraquinone was 1.504%.The extraction efficiency of resveratrol was 90%,and the extraction efficiency of anthraquinone was 91.15%.Acetone:ether 1∶ 3 was used in purifying,then 50% ethanol was used to separate.Resveratrol and anthraquinone content in coarse product was 63.72% and 50.82%,respectively.This study provided experimental evidence for comprehensive development and utilization of Polygonum cuspidatum Sieb.et.Zucc.