To detect protective effect of crude extracts from cold-induced Carrots on Lactobacillus acidophilus,crude protein extracts from cold-induced Carrots were prepared for SDS-PAGE analysis.Six formulas for cryoprotectants were designed,and protective effect of crude extracts were detected with survival rate as index.The morphological characteristics of freeze-dried bacteria powder were observed under the scanning electron microscopy(SEM).The results showed that two bands from crude extracts of cold-induced carrots were found at 12.5 kD and 36 kD.There were significant differences among the protective effects of different protectants on freeze-dried lactobacillus acidophilus.After addition of such crude extracts into cryoprotective agents,the survival rate could reach 76.02%.The best protectants formula was as follows:10% skim milk,10% trehalose,10% maltodextrin,1.0% L-Cys,2.0% VC,2.0% glycerin,0.8% manganese sulphate,and 15% crude extracts from cold-induced Carrots.