Brewer's lager yeast strain TS-01(Saccharomyces pastorianus) was selected as parent strain for directed breeding with resistance induction on medium containing ethionine and hydroxynorvaline successively.Mutant stains with high SO2 and low H2S production were obtained by screening with medium containing lead nitrate.The mutants were fermented in EBC tube and 100L tank for pilot fermentation respectively using the content of SO2,H2S,diacetyl,aldehyde,higher alcohols and the fermentation degree as the selecting indexes.As a result,TK-10 was obtained as strain with optimal fermentation performance.Industrial fermentation was conducted in 600T tank with the parent strain TS-01 as a control.The results showed that TK-10 was obviously increased in the production of SO2 with improved antioxygenic property of beer product.In addition,the beer flavor was maintained with good sensory evaluation.