A new method of monolayer cultivation coupled with double-layer color development was established.96 strains of biogenic amines-producing lactic acid bacteria and 58 strains of biogenic amines-producing Enterobacteria were obtained.The 154 strains of biogenic amines-producing bacteria belonged to 5 species according to the results of polymerase chain reaction denaturing gradient gel eleetrophoresis(PCR-DGGE) analysis.After sequencing of PCR-products of 16S rDNA fragments and alignment in Genebank,the 5 strains of biogenic amines-producing bacteria were found to be Enterococcus faecium,Enterococcus faecalis,Enterobacter cloacae,Escherichia coli,and Enterobacter aerogenes.The presence of Biogenic amines-producing gene was demonstrated by special primers.