To study different components of tea polysaccharides and their anticoagulant activity,the tea polysaccharides were extracted by water and precipitated by alcohol,and purified by ion exchange chromatograph on DEAE sepharose CL-6B column,and its molecular weight was determined by high performance gel permeation chromatograph.The anticoagulant activity of polysaccharide was also studied.The results showed polyamide method was effective on decolorization and deproteination,the decolorization and deproteination and polysaccharides retention rate were 75.5%,91.2% and 79.2% respectively;four componets were obtained after purification: TPS-1,TPS-2,TPS-3 and TPS-4.HPGPC method showed that the TPS-1,TPS-2 and TPS-3 were homogeneous polysaccharides.Their molecular weight were 20760、24230 and 250643,separately,they are homogeneous.TPS-4 has two components of molecular weight of 689113 and 4150,and it was heterogeneous.Anticoagulant activities in vitro tests showed TPS-4 could prolong activated partial thromboplastin time(APTT) and thrombin time(TT).The result indicated TPS in the regulation of coagulation initiated via the intrinsic pathway.