Pleurotus ferulae NB-1 was used to be liquid fermented in this work.After 4 days of fermentation at 25℃,fermentation broth was separated at 5℃ and 25℃ for 1.5 days,then 25℃ for 2 days.Using headspace solid phase micro-extraction and GC/MS/MS,45 kinds and 23 kinds of volatile components were detected in group fermented at 5 and 25℃,respectively.16 volatile components as follows were the same in the two treatment groups: 1-Tetradecanol,1-Undecanol,1-Pentanol,1-Octanol,Benzaldehyde,Hexanal,Nonanal,Undecanal,Acetaldehyde,2-Methylpentanal,2,3-Butanedione,2-Hexanone 2-methyl-Cyclopentanone,3-Methyl-3-oxetanemethanol,2,2,5,5-tetramethyl-3-hexene,and 1-Tridecene.The number of alcohols and aldehydes in group fermented at 25℃ were less 5℃,and acids were not detected.Maybe it will due to the higher temperature which changes the metabolites of Pleurotus ferulae.