Environmental factors such as pH,oxygen content,temperature,addition of NaCl and sugar and effects of amines-productive dominant bacteria on biogenic amines were studied in order to find out production mechanism of phenylethylamine and tyramine by Enterococcus faecium.The results showed that the growth rate and production ability of phenylethylamine and tyramine-production of Enterococcus faecium were inhibited at initial pH 5.00,and production ability of phenylethylamine and tyramine-production was lower at aerobic environment than that at anaerobic ones.Temperature,pH,addition level of NaCl and sugar had significant effect on growth rate of Enterococcus faecium and the effect of pH and temperature was higher by four factors quadratic rotation experiment.There were significant interactional effect of pH and temperature on production of phenylethylamine and tyramine.There were significant interactional effects of addition level of pH and temperature on production of phenylethylamine and tyramine and the effects were also increased with the increase of temperature.