The flavonoids from Lotus leaves were extracted by different methods in order to select the best one.DPPH radical scavenging assay was used to explore their antioxidant ability.Based on median effect principle,the antioxodation effects for Lotus leaf flavonoids solely and combined with Vitamin C were tested.The results showed that the acetone extraction was the best with the highest extraction rate and purity.The IC50 of Lotus leaf flavonoids and VC were 92.89 μg/mL and 1 429.33 μg/mL.The synergistic effect showed when flavonoid concentrations was less than 138.05 g/mL and VC concentrations less than 9.2 μg/mL.