The study focused on a kind of high-quality yeast extract processing technology based on spent brewer's yeast.The process included removal of impurity,debittering,enzymolysis,concentration and spray dehydration,after which a kind of light yellow yeast extract powder with well water-solubility can be obtained.During the process,71.00% of raw yeast's total amount,which be measured by absolutely dry weight,came into the final product,and the percent for crude protein was 85.55%.Analysis result of the product showed that 100 g yeast extract contained 52.90 g crude protein,26.48 g Carbohydrate,0.20 g fat,11.08 g ash and 9.34 g dietary fiber.What's more,free amino acids and oligopeptide was the main form of nitrogen in the yeast extract,and it will be of great important potential material for health-care food.