The high-temperature screening and traditional culture methods combined with ARDRA patterns of the amplified 16S rDNA and phylogenetic analysis were used based on the partial 16S rDNA sequences to explore the thermophilic bacterial community in high temperature Daqu of sesame flavor liquor.The results indicated that the 85 thermophilic bacterias from high temperature Daqu were clustered into 4 genus,Thermoactinomyces sp.,Bacillus sp.,Schlegelella sp.,and Streptomyces sp..Thermoactinomyces vulgaris(69.41% in the group) was the dominant bacteria in the liquor Daqu.The research firstly showed the diversity of thermophilic bacterial community occurred in high temperature Daqu of sesame flavor liquor,which provided the foundation to explore their functionality and to establish their relationship with the flavor of sesame flavor liquor.