The research evaluated the effects of high pressure(HP)treatment on the diffusion of β-cyclodextrin(β-CD)into rice grain.β-CD with concentration of 2%(w/w) was added into the rice and soaked at 60 ℃ for 30min,then the rice was treated by HP at different pressures(100~500 MPa) for 20 min,finally the rice was fully cooked by steam.The residue β-CD content in soaking liquid was quantified by High Pressure Liquid Chromatography(HPLC).The results showed that β-CD content in the soaking liquid was significantly reduced with the increase of pressure.This indicated that more β-CD penetrated into the interior of the rice.The 1,8-two hydroxylanthraquinone was embedded by β-CD to trace the immersion process into the rice grain under high pressure.The fluorescence inclusion distribution of rice after soaking and HP treatment was observed by the confocal laser microscopy.The results showed that HP treatment can effectively increase β-cyclodextrin penetration into the interior of the rice.