Evaluation of immuneactivity a hydrolysate proteolysis from corn gluten meal

  • GAO Jian ,
  • LIN Wei ,
  • LIU Xiaolan ,
  • LIU Xiang ,
  • ZHENG Xiqun ,
  • LI Guanlong
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  • 1(College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China)
    2(Key Laboratory of Corn Deep Processing Theory and Technology, Qiqihar 161006, China)
    3(College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)

Received date: 2020-07-01

  Revised date: 2020-07-14

  Online published: 2021-02-03

Abstract

In order to expand the application of corn gluten meal(CGM) in the field of functional food, the effects of corn gluten hydrolysate (CGH) on spleen lymphocyte proliferation in vitro, immune regulation and antioxidant activities in immunosuppressed mice were studied.CGH was obtained from CGM by synergistic hydrolysis catalyzed by protamex and alcalase.The immunoactivity of CGH was determined by the proliferation of spleen lymphocytes in vitro, then, an immunosuppressive mouse model was constructed.Mice were given CGH by gavage and obtained with cyclophosphamide at twenty-first day.Cytokines (TNF-α, IFN-γ, IL-2 and IL-6), the antioxidant enzymes activities (SOD, GSH-Px, CAT) and malondialdehyde (MDA) content were measured.The results showed that CGH at concentration between 80 μg/mL and 320 μg/mL could promote the proliferation of spleen cells cultured in vitro.Compared to the model group, the high, medium and low doses [200, 100, 50 mg/(kg·d)] could significantly increase the content of TNF-α and IFN-γ;the high and medium doses could significantly increase the content of IL-2;the high dose could significantly increase the content of IL-6 (P<0.05).The high and medium doses [200, 100 mg/(kg·d)] could significantly increase the contents of SOD, GSH-Px, CAT and could significantly decrease the content of MDA (P<0.05).Therefore, CGH has a good immunomodulatory effect in vitro and in vivo, and is a potential immunomodulator, which lays a foundation for the application of immunomodulatory peptides from corn gluten meal in functional foods.

Cite this article

GAO Jian , LIN Wei , LIU Xiaolan , LIU Xiang , ZHENG Xiqun , LI Guanlong . Evaluation of immuneactivity a hydrolysate proteolysis from corn gluten meal[J]. Food and Fermentation Industries, 2021 , 47(1) : 148 -154 . DOI: 10.13995/j.cnki.11-1802/ts.024921

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