The sensory-directed analysis method was used to analyse the aroma-active components of Lanling Meijiu, a northern style Chinese rice wine. Firstly, the aroma profile was constructed by quantitative descriptive analysis. The results showed that Lanling Meijiu had strong soy aroma, floral, alcoholic and caramel notes. A total of 50 aroma compounds were identified by headspace solid phase microextraction and liquid-liquid extraction combined with gas chromatography-olfactometry-mass spectrometry (GC-O-MS), including esters, alcohols, acids, phenols, aldehydes and ketones. Based on the precise quantification and odor activity value (OAV) calculation, ethyl acetate, ethyl butanoate, ethyl valerate, ethyl hexanoate, ethyl octoate, isoamyl alcohol, phenethyl alcohol, benzaldehyde, phenylacetaldehyde, vanillin, γ-nonalactone and 2-methoxy-4-vinylphenol could be potentially important to the overall aroma profile of Lanling Meijiu.
[1] 吕金山,刘剀.兰陵保健美酒的酿造工艺[J].酿酒,1996(2):19.
LU J S,LIU K.The brewing process of Lanling health wine[J].Liquor Making,1996(2):19.
[2] 王丽华,李建飞,王异静,等.GC-O吸闻技术在黄酒风味分析中的应用[J].中国酿造,2010,29(10):158-161.
WANG L H,LI J F,WANG Y J,et al.Application of GC-O technique in flavor analysis of Chinese rice wines[J].China Brewing,2010,29(10):158-161.
[3] 江伟,兰玉倩,黄毅,等.固相微萃取与气相色谱-质谱联用法对不同酒龄黄酒的微量风味分析与应用[J].食品与发酵工业,2011,37(2):144-150.
JIANG W,LAN Y Q,HUANG Y,et al.Analysis and application of trace compounds in rice wine by solid-phase micro etraction and gas chromatograph-mass spectrum[J].Food and Fermentation Industries,2011,37(2):144-150.
[4] 罗涛,范文来,徐岩,等.我国江浙沪黄酒中特征挥发性物质香气活力研究[J].中国酿造,2009,28(2):14-19.
LUO T,FAN W L,XU Y,et al.Aroma components in Chinese rice wines from different regions[J].China Brewing,2009,28(2):14-19.
[5] CHEN S,XU Y,QIAN M C.Chromatography-olfactometry,chemical quantitative analysis,and aroma reconstitution[J].Journal of Agricultural and Food Chemistry,2013,61:11 295-11 302.
[6] CHEN S,XU Y,QIAN M C.Comparison of the aromatic profile of traditional and modern types of Huang Jiu (Chinese rice wine) by aroma extract dilution analysis and chemical analysis[J].Flavour and Fragrance Journal,2018,33:263-271.
[7] CHEN S,WANG C,QIAN M C,et al.Characterization of the key aroma compounds in aged Chinese rice wine by comparative aroma extract dilution analysis,quantitative measurements,aroma recombination,and omission studies[J].Journal of Agricultural and Food Chemistry,2019,67(17):4 876-4 884.
[8] 王洪燕,张生万,李美萍,等.GC-MS分析不同地区黄酒中的易挥发成分[J].酿酒科技,2015(9):115-121.
WANG H Y,ZHANG S W,LI M P,et al.Analysis of volatile compounds in yellow rice wine from different producing places by GC-MS[J].Liquor-Making Science and Technology,2015(9):115-121.
[9] 王栋,经斌,徐岩,等.中国黄酒风味感官特征及其风味轮的构建[J].食品科学,2013,34(5):90-95.
WANG D,JING B,XU Y,et al.Sensory flavor characteristics of Chinese yellow rice wine and construction of flavor wheel[J].Food Science,2013,34(5):90-95.
[10] 陈双,陈华蓉,吴群,等.应用顶空固相微萃取-气相色谱质谱技术解析酿造用高梁蒸煮挥发性香气成分[J].食品与发酵工业,2017,43(4):201-207.
CHEN S,CHEN H R,WU Q,et al.Characterization of volatile aroma compounds in cooked sorghum by HS-SPME and GC-MS[J].Food and Fermentation Industries,2017,43(4):201-207.
[11] 郝飞龙,范莹,延莎,等.山西清香型宗酒原酒与成品酒香气成分及其感官分析[J].食品科技,2018,43(3):255-261.
HAO F L,FAN Y,YAN S,et al.Analysis of aroma components and sensory in light aroma type Shanxi Zongjiu of base liquor and finished liquor[J].Food Science and Technology,2018,43(3):255-261.
[12] 栾金水.黄酒中风味物质的研究[J].中国酿造,2002,21(6):21-24.
LUAN J S.Research on the flavor in rice wine[J].China Brewing,2002,21(6):21-24.
[13] 汪建国.黄酒中色、香、味、体的构成和来源浅析[J].中国酿造,2004,23(4):6-10;18.
WANG J G.Analysis of composition and source of color,aroma,taste,type in rice wine[J].China Brewing,2004,23(4):6-10;18.
[14] GROSCH W.Evaluation of the key odorants of foods by dilution experiments,aroma models and omission[J].Chemical Senses,2001,26(5):533-545.
[15] SCHIEBERLE P.Chapter 17-New developments in methods for analysis of volatile flavor compounds and their precursors[J].Characterization of Food,1995,65(7):403-431
[16] 刘峻溪,王俊芳,韩爱芹,等.葡萄酒中酯类物质的生物合成及其影响因素[J].酿酒科技,2016(9):43-47.
LIU J X,WANG J F,HAN A Q,et al.Biosynthesis of ester compounds in grape wine & relative influencing factors[J].Liquor-Making Science and Technology,2016(9):43-47.
[17] MEILGAARD M C.Flavor chemistry of beer:Part II:Flavor and threshold of 239 aroma volatiles[J].Techquartmaster Brewers Assam,1975,12.
[18] PINEAU B,BARBE J C,LEEUWEN C V,et al.Examples of perceptive interactions involved in specific “red-” and “black-berry” aromas in red wines[J].Journal of Agricultural and Food Chemistry,2009,57(9):3 702-3 708.
[19] 沈怡方.白酒生产技术全书[M].北京:中国轻工业出版社,1998,11-15.
SHEN Y F.Baijiu production technology book[M].Beijing:China Light Industry Press,1998,11-15.
[20] 曹雪丹,李二虎,方修贵,等.蓝莓酒主发酵前后挥发性成分变化的GC-MS分析[J].食品与发酵工业,2015,41(3):179-184.
CAO X D,LI E H,FANG X G,et al.GC-MS analysis of volatiles variation in blueberry wine before and after primary fermentation[J].Food and Fermentation Industries,2015,41(3):179-184.
[21] SOARES D C M,GONCALVES C,FERREIRA A,et al.Further insights into the role of methional and phenylacetaldehyde in lager beer flavor stability[J].Journal of Agricultural and Food Chemistry,2004,52(26):7 911-7 917.
[22] 莫新良,徐岩,范文来.中国黄酒中香草醛的定性定量分析[J].食品与发酵工业,2012,38(12):133-137.
MO X L,XU Y,FAN W L.The qualitative and quantitative analysis of vanillin in different Chinese rice wines[J].Food and Fermentation Industries,2012,38(12):133-137.