Food and Fermentation Industries >

2021 , Vol. 47 >Issue 4: 222 - 230

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025855

Ultra-performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry method for rapid screening of bioactive compounds in lemon fruits

  • PANG Wenhui ,
  • ZHAO Xijuan ,
  • CHEN Xi ,
  • ZHANG Yaohai ,
  • WANG Chengqiu ,
  • ZHAO Qiyang ,
  • JIAO Bining
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  • 1(Quality Supervision and Testing Center for Citrus and Seedling, Ministry of Agriculture and Rural Affairs, Laboratory of Risk Assessment for Citrus Quality and Safety, Citrus Research Institute, Chinese Academy of Agricultural Sciences, Southwest University, Chongqing 400712,China)
    2(College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715,China)
    3(SCIEX Analytical Instrument Trading Co.Ltd., Shanghai 200335, China)

Received date: 2020-10-11

  Revised date: 2020-11-09

  Online published: 2021-03-16

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Abstract

Base on the bioactive compounds mass spectrometry database established by our laboratory, this study established an ultra performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-HRMS) method for simultaneous screening and accurate identification of bioactive compounds, such as flavonoids, coumarins, phenolic acids, alkaloids and limonoids, which was further applied for rapid and accurate screening of bioactive compounds of six different parts of lemons (peel, pulp and juice). The result showed that 40 flavonoids, 27 coumarins, 9 phenolic acids, 7 alkaloids and 3 limonoids were determined in the six kinds of lemon samples. Specifically, 72, 71, 70, 66, 64 and 63 kinds of bioactive compounds were identified in the screening of Bianhongningmeng, Beijingningmeng, Keerticuningmeng, Youlikeningmeng, and Lisibenningmeng, and Meiguocuningmeng, respectively. The number of bioactive compounds in different parts showed the following order: peel ≥ pulp ≥ juice. Through actual sample analysis, this method can be used in the identification of bioactive compounds in lemon and other citrus fruits.

Key words： ultra-performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry(UPLC-Q-TOF-HRMS); mass spectrometry database; information-dependent acquisition(IDA); bioactive compounds; lemon

Cite this article

PANG Wenhui , ZHAO Xijuan , CHEN Xi , ZHANG Yaohai , WANG Chengqiu , ZHAO Qiyang , JIAO Bining . Ultra-performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry method for rapid screening of bioactive compounds in lemon fruits[J]. Food and Fermentation Industries, 2021 , 47(4) : 222 -230 . DOI: 10.13995/j.cnki.11-1802/ts.025855

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